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DROSHA RNA-Seq in K562



DROSHA      CRISPR in K562      Control:

General Information
RBPDROSHA
Cell_LineK562
MethodCRISPR
Exp_NameDROSHA-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1DROSHA-BGKcLV42-25
Rep2DROSHA-BGKcLV42-26
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR79.0
Rep2_qPCR78.2
Rep1_WB72.2
Rep2_WB65.0
Antibody Cat#3364S
Antibody Lot#lot # 5
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1536




Experiment Information (Status: Sequenced)
BGKcLV42-25BGKcLV42-26
idx00
TRCN#_or_BGC#BGC#0001127BGC#0001127
shRNA_or_gRNA_sequenceACAGATCTGATTATGACCGAACAGATCTGATTATGACCGA
PAMGGGGGG
NameDROSHA_93DROSHA_93
Sample_IDBGKcLV42-25BGKcLV42-26
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameDROSHADROSHA
qPCR_result79.078.2
Ave_qPCR78.6
RT-qPCR_primer-Faggccagatgaatgatggac
RT-qPCR_primer-Rcttgatggcctcttctccag
protein_conc23642671
WB_result72.265.0
Ave_WB68.6
WB_DONE_date5/6/24
MW160kd
IP
antibody_Cat#3364Slot # 5
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57855786




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DROSHAProduct_ID: 3364s
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: DROSHA-human		 CST_3364s_5_DROSHA.png<br>Caption: IP-WB analysis of 3364s whole cell lysate using the DROSHA specific antibody, 3364s. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DROSHA-specific antibody, 3364S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DROSHA-K562-CRISPR-3364S.png<br>Caption: Western blot following CRISPR against DROSHA in K562 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-25BGKcLV42-26
Sample_IDBGKcLV42-25BGKcLV42-26
Sample Name
Sample NameDROSHA-BGKcLV42-25DROSHA-BGKcLV42-26
RBPDROSHADROSHA
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1306613067




Library-Prep Information
BGKcLV42-25BGKcLV42-26
Sample #2324
Sample NameDROSHA-BGKcLV42-25DROSHA-BGKcLV42-26
Sample_Name_Alias
Index Well PositionG03H03
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp279282
Peak_Molarity72.2082.60
libSampleQC_DNA_WellDNA_Library_set73/set1/G3.pngDNA_Library_set73/set1/H3.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/G3.pngRNA_Library_set73/set1/H3.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-25BGKcLV42-26
RBPDROSHADROSHA
Batch_IDBGKcLV42BGKcLV42
WB_result72.20065.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42924293




Sequencing Information
BGKcLV42-25BGKcLV42-26
Sample_IDBGKcLV42-25BGKcLV42-26
Sample NameDROSHA-BGKcLV42-25DROSHA-BGKcLV42-26
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads37,049,67842,634,381
total_aligned_reads27,945,36035,850,940
unique_aligned_reads25,700,52532,995,770
percent_uniqueAligned0.693680.77392
correlation_replicates0.9922600.992260
spikein_reads4,5365,890
percent_spikeins0.000120.00014
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31593160




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database