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WDR77 RNA-Seq in K562



WDR77      CRISPR in K562      Control:

General Information
RBPWDR77
Cell_LineK562
MethodCRISPR
Exp_NameWDR77-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1WDR77-BGKcLV42-105
Rep2WDR77-BGKcLV42-106
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR30.8
Rep2_qPCR33.6
Rep1_WB49.8
Rep2_WB59.1
Antibody Cat#2018S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1568




Experiment Information (Status: Sequenced)
BGKcLV42-105BGKcLV42-106
idx00
TRCN#_or_BGC#BGC#0001206BGC#0001206
shRNA_or_gRNA_sequenceCAACGTGGTGGACGACCTGACAACGTGGTGGACGACCTGA
PAMTGGTGG
NameWDR77_91WDR77_91
Sample_IDBGKcLV42-105BGKcLV42-106
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameWDR77WDR77
qPCR_result30.833.6
Ave_qPCR32.2
RT-qPCR_primer-Fctgcctctcctcacaaggac
RT-qPCR_primer-Rcagcgggtatcccagagtaa
protein_conc32062841
WB_result49.859.1
Ave_WB54.5
WB_DONE_date5/10/24,5/23/24,6/21
MW42kd
IP
antibody_Cat#2018Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58715872




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
WDR77Product_ID: 2018S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: WDR77-human		 CST_2018S_1_WDR77.png<br>Caption: IP-WB analysis of 2018S whole cell lysate using the WDR77 specific antibody, 2018S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the WDR77-specific antibody, 2018S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 WDR77-K562-CRISPR-2018S.png<br>Caption: Western blot following CRISPR against WDR77 in K562 whole cell lysate using WDR77 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against WDR77. WDR77 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-105BGKcLV42-106
Sample_IDBGKcLV42-105BGKcLV42-106
Sample Name
Sample NameWDR77-BGKcLV42-105WDR77-BGKcLV42-106
RBPWDR77WDR77
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1313013131




Library-Prep Information
BGKcLV42-105BGKcLV42-106
Sample #8788
Sample NameWDR77-BGKcLV42-105WDR77-BGKcLV42-106
Sample_Name_Alias
Index Well PositionG11H11
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp291302
Peak_Molarity86.8084.80
libSampleQC_DNA_WellDNA_Library_set73/set1/G11.pngDNA_Library_set73/set1/H11.png
RIN9.810.0
libSample_RNA_WellRNA_Library_set73/set1/G11.pngRNA_Library_set73/set1/H11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-105BGKcLV42-106
RBPWDR77WDR77
Batch_IDBGKcLV42BGKcLV42
WB_result49.80059.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43564357




Sequencing Information
BGKcLV42-105BGKcLV42-106
Sample_IDBGKcLV42-105BGKcLV42-106
Sample NameWDR77-BGKcLV42-105WDR77-BGKcLV42-106
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads35,213,36233,111,029
total_aligned_reads30,193,84729,004,424
unique_aligned_reads27,749,24026,664,835
percent_uniqueAligned0.788030.80532
correlation_replicates0.9946100.994610
spikein_reads6,1824,692
percent_spikeins0.000180.00014
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32233224




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database