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VASP RNA-Seq in K562



VASP      CRISPR in K562      Control:

General Information
RBPVASP
Cell_LineK562
MethodCRISPR
Exp_NameVASP-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1VASP-BGKcLV42-103
Rep2VASP-BGKcLV42-104
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR45.0
Rep2_qPCR49.6
Rep1_WB98.7
Rep2_WB97.4
Antibody Cat#3132S
Antibody Lot#lot # 5
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1567




Experiment Information (Status: Sequenced)
BGKcLV42-103BGKcLV42-104
idx00
TRCN#_or_BGC#BGC#0001204BGC#0001204
shRNA_or_gRNA_sequenceCGCGAAAGGAATTGGCCGTGCGCGAAAGGAATTGGCCGTG
PAMGGGGGG
NameVASP_96VASP_96
Sample_IDBGKcLV42-103BGKcLV42-104
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameVASPVASP
qPCR_result45.049.6
Ave_qPCR47.3
RT-qPCR_primer-Fcaacctccacaccctcaagt
RT-qPCR_primer-Rgcaatttgctgctttgtgaa
protein_conc28793411
WB_result98.797.4
Ave_WB98.1
WB_DONE_date5/10/24
MW46,50kd
IP
antibody_Cat#3132Slot # 5
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58695870




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
VASPProduct_ID: 3132S
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: VASP-human		 VASP-HEPG2-CRISPR-3132S.png<br>Caption: Western blot following CRISPR against VASP in HepG2 whole cell lysate using VASP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against VASP. VASP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3132S_5_VASP.png<br>Caption: IP-WB analysis of 3132S whole cell lysate using the VASP specific antibody, 3132S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the VASP-specific antibody, 3132S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 VASP-K562-CRISPR-3132S.png<br>Caption: Western blot following CRISPR against VASP in K562 whole cell lysate using VASP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against VASP. VASP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-103BGKcLV42-104
Sample_IDBGKcLV42-103BGKcLV42-104
Sample Name
Sample NameVASP-BGKcLV42-103VASP-BGKcLV42-104
RBPVASPVASP
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1312813129




Library-Prep Information
BGKcLV42-103BGKcLV42-104
Sample #8586
Sample NameVASP-BGKcLV42-103VASP-BGKcLV42-104
Sample_Name_Alias
Index Well PositionE11F11
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp315292
Peak_Molarity86.1085.80
libSampleQC_DNA_WellDNA_Library_set73/set1/E11.pngDNA_Library_set73/set1/F11.png
RIN10.09.9
libSample_RNA_WellRNA_Library_set73/set1/E11.pngRNA_Library_set73/set1/F11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-103BGKcLV42-104
RBPVASPVASP
Batch_IDBGKcLV42BGKcLV42
WB_result98.70097.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43544355




Sequencing Information
BGKcLV42-103BGKcLV42-104
Sample_IDBGKcLV42-103BGKcLV42-104
Sample NameVASP-BGKcLV42-103VASP-BGKcLV42-104
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads41,801,85730,100,370
total_aligned_reads39,294,87324,441,267
unique_aligned_reads36,281,51022,439,651
percent_uniqueAligned0.867940.74549
correlation_replicates0.9949540.994954
spikein_reads2,326590
percent_spikeins0.000060.00002
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32213222




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database