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SF3B3
CRISPR
in K562
Control:
NT-BGKcLV33
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
SF3B3
Cell_Line
K562
Method
CRISPR
Exp_Name
SF3B3-BGKcLV33-K562
ENCODE_series_ID
ENCSR497ASE
Batch_ID
BGKcLV33
Pool ID
Pool-220418
Local_Set_Name
set53
ENCODE_KD_Exp_ID
ENCSR034FLW
ENCODE_CN_Exp_ID
ENCSR271ZAB
Rep1
SF3B3-BGKcLV33-53
Rep2
SF3B3-BGKcLV33-54
CN1
NT-BGKcLV33-1
CN2
NT-BGKcLV33-2
Rep1_qPCR
Rep2_qPCR
Rep1_WB
no band on the position
Rep2_WB
Antibody Cat#
A302-170A
Antibody Lot#
LOT #1
Antibody DCC ID
Status
Submitted
Project
ENCODE4
ID
908
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGKcLV33-47
BGKcLV33-47
BGKcLV33-48
BGKcLV33-48
idx
1149
1236
1150
1237
TRCN#_or_BGC#
BGC#0000865
BGC#0000859
BGC#0000865
BGC#0000859
shRNA_or_gRNA_sequence
ACTGGCCAGGAACGATGCGA
TGAATTCACGCTTATGGTAG
ACTGGCCAGGAACGATGCGA
TGAATTCACGCTTATGGTAG
PAM
CGG
TGG
CGG
TGG
Name
SF3B3_95
SEPTIN7_90
SF3B3_95
SEPTIN7_90
Sample_ID
BGKcLV33-47
BGKcLV33-47
BGKcLV33-48
BGKcLV33-48
transduction_Date
12/7/21
2/22/22
12/7/21
2/22/22
days
D6
D6
D6
D6
RBP_name
SF3B3
SEPT7
SF3B3
SEPT7
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc
448
2104
548
2409
WB_result
0.0
0.0
0.0
0.0
Ave_WB
0.0
0.0
WB_DONE_date
2/3/22
3/14/22
MW
136kd
51kd
IP
antibody_Cat#
A302-508A
A304-212A
LOT #1
LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
0
0
0
0
Rep2_TPM
0
0
0
0
Action
Library_start_date
repeat_library
Note
ID
5289
5378
5290
5379
Western Blot
Western Blot info was not avaliable
Experiment Status
BGKcLV33-47
BGKcLV33-47
BGKcLV33-48
BGKcLV33-48
Sample_ID
BGKcLV33-47
BGKcLV33-47
BGKcLV33-48
BGKcLV33-48
Sample Name
Sample Name
RBP
SF3B3
SEPT7
SF3B3
SEPT7
Cell_Line
K562
K562
K562
K562
Exp UID
Status
NotSatisfied
NotSatisfied
NotSatisfied
NotSatisfied
Status_date
2022-03-30
2022-03-30
2022-03-30
2022-03-30
Project
ENCODE4
ENCODE4
ENCODE4
ENCODE4
Note
ID
10733
10783
10734
10784
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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