MORC2 RNA-Seq in K562

General Information


RBP	MORC2
Cell_Line	K562
Method	CRISPR
Exp_Name	MORC2-BGKcLV19-K562
ENCODE_series_ID	ENCSR702HJT
Batch_ID	BGKcLV19
Pool ID	Pool-200928
Local_Set_Name	set45_K
ENCODE_KD_Exp_ID	ENCSR990DKW
ENCODE_CN_Exp_ID	ENCSR978MMZ
Rep1	MORC2-BGKcLV19-23
Rep2	MORC2-BGKcLV19-24
CN1	NT-BGKcLV19-1
CN2	NT-BGKcLV19-2
Rep1_qPCR	0
Rep2_qPCR	0
Rep1_WB	73.8
Rep2_WB	85.0
Antibody Cat#	PA5-51172
Antibody Lot#	lot#UF2796314
Antibody DCC ID	ENCAB179RUB
Status	Released
Project	ENCODE4
ID	53

Experiment Information (Status: Released)

	BGKcLV19-23	BGKcLV19-24
idx	695	696
TRCN#_or_BGC#	BGC#0000471	BGC#0000471
shRNA_or_gRNA_sequence	AGATTGGGCAGTACGGGAAT	AGATTGGGCAGTACGGGAAT
PAM	GGG	GGG
Name	MORC2_91	MORC2_91
Sample_ID	BGKcLV19-23	BGKcLV19-24
transduction_Date	11/6/19	11/6/19
days	Day 6	Day 6
RBP_name	MORC2	MORC2
qPCR_result	0	0
Ave_qPCR	0.0
RT-qPCR_primer-F	GCAGAGAAGCCGATGTCAAG
RT-qPCR_primer-R	CAGATCCCGGTGTTCAATGT
protein_conc	4275	4708
WB_result	73.8	85.0
Ave_WB	79.4
WB_DONE_date	8/5/20	jess
MW	117.8kd
IP	Rabbit / I
antibody_Cat#	PA5-51172	lot#UF2796314
Antibody DCC ID	ENCAB179RUB
submitted_to_DCC_date	11/16/20
Rep1_TPM	22.5	0
Rep2_TPM	21.6	0
Action	Ready	Ready
Library_start_date	09/24/20	09/24/20
repeat_library
Note
ID	4815	4816

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
MORC2	Product_ID: PA5-51172 Lot_ID: UF2796314 Source: Thermo Fisher Target Name: MORC2-human

Experiment Status

	BGKcLV19-23	BGKcLV19-24
Sample_ID	BGKcLV19-23	BGKcLV19-24
Sample Name
Sample Name	MORC2-BGKcLV19-23	MORC2-BGKcLV19-24
RBP	MORC2	MORC2
Cell_Line	K562	K562
Exp UID
Status	Released	Released
Status_date	2021-03-18	2021-03-18
Project	ENCODE4	ENCODE4
Note
ID	5699	5700

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV19-23	BGKcLV19-24
Sample #	13	14
Sample Name	MORC2-BGKcLV19-23	MORC2-BGKcLV19-24
Sample_Name_Alias
Index Well Position	G01	H01
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2020-09-24	2020-09-24
Lib_ID	Lib-200924	Lib-200924
Tecan_Location
Tecan
Tecan_date
Size_bp	304	300
Peak_Molarity	88.50	94.80
libSampleQC_DNA_Well	DNA_Library_set45/set1/G1.png	DNA_Library_set45/set1/H1.png
RIN	9.5	9.5
libSample_RNA_Well	RNA_Library_set45/set1/G1.png	RNA_Library_set45/set1/H1.png
SampleQC_method	TapeStation	TapeStation
SampleQC_date	2020-09-22	2020-09-22
Sample_ID	BGKcLV19-23	BGKcLV19-24
RBP	MORC2	MORC2
Batch_ID	BGKcLV19	BGKcLV19
WB_result	73.800	85.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCODE4	ENCODE4
ID	787	788

Sequencing Information

	BGKcLV19-23	BGKcLV19-24
Sample_ID	BGKcLV19-23	BGKcLV19-24
Sample Name	MORC2-BGKcLV19-23	MORC2-BGKcLV19-24
Pool ID	Pool-200928	Pool-200928
LocalServer_folder	set45_K	set45_K
total_reads	104,684,172	96,057,258
total_aligned_reads	97,449,336	89,144,858
unique_aligned_reads	88,976,078	81,595,990
percent_uniqueAligned	0.84995	0.84945
correlation_replicates	0.990722	0.990722
spikein_reads	84,098	112,748
percent_spikeins	0.00080	0.00117
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	418	419

Data Submission Information

	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-24	BGKcLV19-24	BGKcLV19-24	BGKcLV19-24
ENCODE_alias	brenton-graveley:MORC2-BGKcLV19	brenton-graveley:MORC2-BGKcLV19-23	brenton-graveley:L-MORC2-BGKcLV19-23	brenton-graveley:MORC2-BGKcLV19-23_S7_R1_001.fastq.gz	brenton-graveley:MORC2-BGKcLV19-23_S7_R2_001.fastq.gz	brenton-graveley:MORC2-BGKcLV19-K562	brenton-graveley:MORC2-BGKcLV19-24	brenton-graveley:L-MORC2-BGKcLV19-24	brenton-graveley:MORC2-BGKcLV19-24_S8_R1_001.fastq.gz	brenton-graveley:MORC2-BGKcLV19-24_S8_R2_001.fastq.gz
ENCODE_accession	ENCSR990DKW	ENCBS293GUO	ENCLB902VRH	ENCFF722YZF	ENCFF074DWR	ENCSR702HJT	ENCBS715XCC	ENCLB750LGG	ENCFF330WER	ENCFF037PTG
object_type	experiment	biosample	library	file_fastq1	file_fastq2	gene_silencing_series	biosample	library	file_fastq1	file_fastq2
assay_type	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq
Sample_ID	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-23	BGKcLV19-24	BGKcLV19-24	BGKcLV19-24	BGKcLV19-24
Sample Name	MORC2-BGKcLV19-23	MORC2-BGKcLV19-23	MORC2-BGKcLV19-23	MORC2-BGKcLV19-23	MORC2-BGKcLV19-23	MORC2-BGKcLV19-23	MORC2-BGKcLV19-24	MORC2-BGKcLV19-24	MORC2-BGKcLV19-24	MORC2-BGKcLV19-24
Selected	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Status	Released	Released	Released	Released	Released	Released	Released	Released	Released	Released
Status_Date	2021-03-18	2021-03-18	2021-03-18	2021-03-18	2021-03-18	2022-03-02	2021-03-18	2021-03-18	2021-03-18	2021-03-18
Project	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4
		LV45K_biosample_protocol	LV45K_library_protocol				LV45K_biosample_protocol	LV45K_library_protocol
protocol_URL		MORC2-BGKcLV19-23.pdf	L-MORC2-BGKcLV19-23.pdf				MORC2-BGKcLV19-24.pdf	L-MORC2-BGKcLV19-24.pdf
ID	2093	2141	2211	2281	2351	7260	2142	2212	2282	2352

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
MORC2-BGKcLV19-23_S7_R1_001.fastq.gz	ENCODE_DATA/set45_K/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	418	NovaSeq6000	100	paired-ended	1				NT-BGKcLV19-1_S1_R1_001.fastq.gz,NT-BGKcLV19-2_S2_R1_001.fastq.gz	ENCODE4	1230
MORC2-BGKcLV19-23_S7_R2_001.fastq.gz	ENCODE_DATA/set45_K/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	418	NovaSeq6000	100	paired-ended	2				NT-BGKcLV19-1_S1_R2_001.fastq.gz,NT-BGKcLV19-2_S2_R2_001.fastq.gz	ENCODE4	1280
MORC2-BGKcLV19-23_quant.sf	ENCODE_DATA/set45_K/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23_S7_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGKcLV19-23_S7_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	25223
MORC2-BGKcLV19-23_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23_S7_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGKcLV19-23_S7_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	27177
MORC2-BGKcLV19-23_Signal.Unique.strand+.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23/Aligned.sortedByCoord.out.bam		ENCORE2	27227
MORC2-BGKcLV19-23_Signal.Unique.strand-.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23/Aligned.sortedByCoord.out.bam		ENCORE2	27277
MORC2-BGKcLV19-23_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23/Aligned.sortedByCoord.out.bam		ENCORE2	27327
MORC2-BGKcLV19-23_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	418					GRCh38	V40	MORC2-BGKcLV19-23/Aligned.sortedByCoord.out.bam		ENCORE2	27377
MORC2-BGKcLV19-24_S8_R1_001.fastq.gz	ENCODE_DATA/set45_K/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	419	NovaSeq6000	100	paired-ended	1				NT-BGKcLV19-1_S1_R1_001.fastq.gz,NT-BGKcLV19-2_S2_R1_001.fastq.gz	ENCODE4	1231
MORC2-BGKcLV19-24_S8_R2_001.fastq.gz	ENCODE_DATA/set45_K/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	419	NovaSeq6000	100	paired-ended	2				NT-BGKcLV19-1_S1_R2_001.fastq.gz,NT-BGKcLV19-2_S2_R2_001.fastq.gz	ENCODE4	1281
MORC2-BGKcLV19-24_quant.sf	ENCODE_DATA/set45_K/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24_S8_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGKcLV19-24_S8_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	25224
MORC2-BGKcLV19-24_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24_S8_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGKcLV19-24_S8_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	27178
MORC2-BGKcLV19-24_Signal.Unique.strand+.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24/Aligned.sortedByCoord.out.bam		ENCORE2	27228
MORC2-BGKcLV19-24_Signal.Unique.strand-.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24/Aligned.sortedByCoord.out.bam		ENCORE2	27278
MORC2-BGKcLV19-24_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24/Aligned.sortedByCoord.out.bam		ENCORE2	27328
MORC2-BGKcLV19-24_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set45_K/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	419					GRCh38	V40	MORC2-BGKcLV19-24/Aligned.sortedByCoord.out.bam		ENCORE2	27378