EIF4E      CRISPR in K562      Control: NT-BGKcLV15

General Information
RBPEIF4E
Cell_LineK562
MethodCRISPR
Exp_NameEIF4E-BGKcLV15-K562
ENCODE_series_IDENCSR173LAM
Batch_IDBGKcLV15
Pool IDPool-200102
Local_Set_Nameset43_K
ENCODE_KD_Exp_IDENCSR033XYV
ENCODE_CN_Exp_IDENCSR404OHQ
Rep1EIF4E-BGKcLV15-33
Rep2EIF4E-BGKcLV15-34
CN1NT-BGKcLV15-1
CN2NT-BGKcLV15-2
Rep1_qPCR68.1
Rep2_qPCR50.2
Rep1_WB95.6
Rep2_WB94.5
Antibody Cat#MA1-089
Antibody Lot#LOT# TD257774
Antibody DCC IDENCAB864VOP
StatusReleased
ProjectENCODE4
ID127




Experiment Information (Status: NotSatisfied)
BGKcLV15-35BGKcLV15-36
idx573574
TRCN#_or_BGC#BGC#0000350BGC#0000350
shRNA_or_gRNA_sequenceCAAACTTGGAGATCAGCCGCCAAACTTGGAGATCAGCCGC
PAMAGGAGG
NameEIF4E-83EIF4E-83
Sample_IDBGKcLV15-35BGKcLV15-36
transduction_Date4/2/194/2/19
daysDay 6Day 6
RBP_nameEIF4EEIF4E
qPCR_result26.924.9
Ave_qPCR25.9
RT-qPCR_primer-FAGAAAAACAAACGGGGAGGA
RT-qPCR_primer-RTCAAAAGATTCTCCAATAAGGCA
protein_conc49155294
WB_result76.366.9
Ave_WB71.6
WB_DONE_date4/9/19
MW25KD
IPMouse / Ig
antibody_Cat#MA1-089
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM116.70
Rep2_TPM130.10
Action
Library_start_date
repeat_library
Note
ID46854686




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV15-35BGKcLV15-36
Sample_IDBGKcLV15-35BGKcLV15-36
Sample Name
Sample Name
RBPEIF4EEIF4E
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2020-09-122020-09-12
ProjectENCODE4ENCODE4
Note
ID53995400




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database