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EIF4A3
CRISPR
in K562
Control:
NT-BGKcLV15
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
EIF4A3
Cell_Line
K562
Method
CRISPR
Exp_Name
EIF4A3-BGKcLV15-K562
ENCODE_series_ID
ENCSR635OQT
Batch_ID
BGKcLV15
Pool ID
Pool-200102
Local_Set_Name
set43_K
ENCODE_KD_Exp_ID
ENCSR264MSX
ENCODE_CN_Exp_ID
ENCSR404OHQ
Rep1
EIF4A3-BGKcLV15-31
Rep2
EIF4A3-BGKcLV15-32
CN1
NT-BGKcLV15-1
CN2
NT-BGKcLV15-2
Rep1_qPCR
0.0
Rep2_qPCR
2.0
Rep1_WB
93.2
Rep2_WB
81.8
Antibody Cat#
PA5-54532
Antibody Lot#
LOT#TK2671524A
Antibody DCC ID
ENCAB214HYQ
Status
Released
Project
ENCODE4
ID
126
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGKcLV15-29
BGKcLV15-30
idx
565
566
TRCN#_or_BGC#
BGC#0000347
BGC#0000347
shRNA_or_gRNA_sequence
GCCCATGGTGTCGAACGTGG
GCCCATGGTGTCGAACGTGG
PAM
GGG
GGG
Name
EIF4A3-94
EIF4A3-94
Sample_ID
BGKcLV15-29
BGKcLV15-30
transduction_Date
4/2/19
4/2/19
days
Day 6
Day 6
RBP_name
EIF4A3
EIF4A3
qPCR_result
0.0
24.0
Ave_qPCR
12.0
RT-qPCR_primer-F
GAATTCGAGACCAGCGAGGA
RT-qPCR_primer-R
GCTGGATTGCTGATGGTTTT
protein_conc
4142
4278
WB_result
38.2
27.6
Ave_WB
32.9
WB_DONE_date
4/18/19
MW
47KD
IP
Rabbit / I
antibody_Cat#
PA5-54532
LOT#TK2671524A
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
89.1
0
Rep2_TPM
79.2
0
Action
Library_start_date
repeat_library
Note
ID
4675
4676
Western Blot
Western Blot info was not avaliable
Experiment Status
BGKcLV15-29
BGKcLV15-30
Sample_ID
BGKcLV15-29
BGKcLV15-30
Sample Name
Sample Name
RBP
EIF4A3
EIF4A3
Cell_Line
K562
K562
Exp UID
Status
NotSatisfied
NotSatisfied
Status_date
2020-09-12
2020-09-12
Project
ENCODE4
ENCODE4
Note
ID
5395
5396
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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