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EIF2AK1
CRISPR
in K562
Control:
NT-BGKcLV15
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
EIF2AK1
Cell_Line
K562
Method
CRISPR
Exp_Name
EIF2AK1-BGKcLV15-K562
ENCODE_series_ID
ENCSR340GYM
Batch_ID
BGKcLV15
Pool ID
Pool-200102
Local_Set_Name
set43_K
ENCODE_KD_Exp_ID
ENCSR667WZB
ENCODE_CN_Exp_ID
ENCSR404OHQ
Rep1
EIF2AK1-BGKcLV15-23
Rep2
EIF2AK1-BGKcLV15-24
CN1
NT-BGKcLV15-1
CN2
NT-BGKcLV15-2
Rep1_qPCR
77.2
Rep2_qPCR
74.6
Rep1_WB
79.0
Rep2_WB
75.2
Antibody Cat#
PA5-29409
Antibody Lot#
LOT# TK267536A
Antibody DCC ID
ENCAB472KCD
Status
Released
Project
ENCODE4
ID
124
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGKcLV15-21
BGKcLV15-21
BGKcLV15-22
BGKcLV15-22
idx
553
557
554
558
TRCN#_or_BGC#
BGC#0000346
BGC#0000346
BGC#0000346
BGC#0000346
shRNA_or_gRNA_sequence
ATGAACATGTTCTATCCACG
ATGAACATGTTCTATCCACG
ATGAACATGTTCTATCCACG
ATGAACATGTTCTATCCACG
PAM
cgg
cgg
cgg
cgg
Name
EIF2AK1-89
EIF2AK1-89
EIF2AK1-89
EIF2AK1-89
Sample_ID
BGKcLV15-21
BGKcLV15-21
BGKcLV15-22
BGKcLV15-22
transduction_Date
4/2/19
4/2/19
4/2/19
4/2/19
days
Day 6
Day 6
Day 6
Day 6
RBP_name
EIF2AK1
EIF2AK1
EIF2AK1
EIF2AK1
qPCR_result
68.5
75.0
Ave_qPCR
71.8
RT-qPCR_primer-F
TTGCCATCTCTGGAAGTGCT
RT-qPCR_primer-R
CGTTTTTCTTTTTCTGGGGTG
protein_conc
6000
6000
5790
5790
WB_result
75.6
NO CLEAR BAND
44.2
Ave_WB
59.9
WB_DONE_date
4/10/19,4/11/19,4/30
4/10/19,4/11/19,4/30
WES, LICOR
WES, LICOR
MW
71KD
71KD
IP
Rabbit / I
Rabbit / I
antibody_Cat#
PA5-29409
711581
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
94.1
94.1
0
0
Rep2_TPM
102.6
102.6
0
0
Action
Library_start_date
repeat_library
Note
ID
4663
4667
4664
4668
Western Blot
Western Blot info was not avaliable
Experiment Status
BGKcLV15-21
BGKcLV15-22
Sample_ID
BGKcLV15-21
BGKcLV15-22
Sample Name
Sample Name
RBP
EIF2AK1
EIF2AK1
Cell_Line
K562
K562
Exp UID
Status
NotSatisfied
NotSatisfied
Status_date
2020-09-12
2020-09-12
Project
ENCODE4
ENCODE4
Note
ID
5389
5390
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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