EIF2AK1      CRISPR in K562      Control: NT-BGKcLV15

General Information
RBPEIF2AK1
Cell_LineK562
MethodCRISPR
Exp_NameEIF2AK1-BGKcLV15-K562
ENCODE_series_IDENCSR340GYM
Batch_IDBGKcLV15
Pool IDPool-200102
Local_Set_Nameset43_K
ENCODE_KD_Exp_IDENCSR667WZB
ENCODE_CN_Exp_IDENCSR404OHQ
Rep1EIF2AK1-BGKcLV15-23
Rep2EIF2AK1-BGKcLV15-24
CN1NT-BGKcLV15-1
CN2NT-BGKcLV15-2
Rep1_qPCR77.2
Rep2_qPCR74.6
Rep1_WB79.0
Rep2_WB75.2
Antibody Cat#PA5-29409
Antibody Lot#LOT# TK267536A
Antibody DCC IDENCAB472KCD
StatusReleased
ProjectENCODE4
ID124




Experiment Information (Status: NotSatisfied)
BGKcLV15-21BGKcLV15-21BGKcLV15-22BGKcLV15-22
idx553557554558
TRCN#_or_BGC#BGC#0000346BGC#0000346BGC#0000346BGC#0000346
shRNA_or_gRNA_sequenceATGAACATGTTCTATCCACGATGAACATGTTCTATCCACGATGAACATGTTCTATCCACGATGAACATGTTCTATCCACG
PAMcggcggcggcgg
NameEIF2AK1-89EIF2AK1-89EIF2AK1-89EIF2AK1-89
Sample_IDBGKcLV15-21BGKcLV15-21BGKcLV15-22BGKcLV15-22
transduction_Date4/2/194/2/194/2/194/2/19
daysDay 6Day 6Day 6Day 6
RBP_nameEIF2AK1EIF2AK1EIF2AK1EIF2AK1
qPCR_result68.575.0
Ave_qPCR71.8
RT-qPCR_primer-FTTGCCATCTCTGGAAGTGCT
RT-qPCR_primer-RCGTTTTTCTTTTTCTGGGGTG
protein_conc6000600057905790
WB_result75.6NO CLEAR BAND44.2
Ave_WB59.9
WB_DONE_date4/10/19,4/11/19,4/304/10/19,4/11/19,4/30WES, LICORWES, LICOR
MW71KD71KD
IPRabbit / IRabbit / I
antibody_Cat#PA5-29409711581
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM94.194.100
Rep2_TPM102.6102.600
Action
Library_start_date
repeat_library
Note
ID4663466746644668




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV15-21BGKcLV15-22
Sample_IDBGKcLV15-21BGKcLV15-22
Sample Name
Sample Name
RBPEIF2AK1EIF2AK1
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2020-09-122020-09-12
ProjectENCODE4ENCODE4
Note
ID53895390




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database