EIF2A      CRISPR in K562      Batch: BGKcLV15

Experiment Information (Status: NotSatisfied)
BGKcLV15-19BGKcLV15-19BGKcLV15-20BGKcLV15-20
idx547551548552
TRCN#_or_BGC#BGC#0000381BGC#0000381BGC#0000381BGC#0000381
shRNA_or_gRNA_sequenceCTATAGTAGGCTGCATTACGCTATAGTAGGCTGCATTACGCTATAGTAGGCTGCATTACGCTATAGTAGGCTGCATTACG
PAMaggaggaggagg
NameEIF2A_96EIF2A_96EIF2A_96EIF2A_96
Sample_IDBGKcLV15-19BGKcLV15-19BGKcLV15-20BGKcLV15-20
transduction_Date4/2/194/2/194/2/194/2/19
daysDay 6Day 6Day 6Day 6
RBP_nameEIF2AEIF2AEIF2AEIF2A
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc5949594960736073
WB_resultNeed discussionNo clear band on the position
Ave_WB
WB_DONE_date4/10/19,4/30/195/10/19wes, licorwes
MW65KD
IPMouse / IgRabbit / IEIF2A pSer
antibody_Cat#AHO0802710292
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM175.7175.700
Rep2_TPM249.9249.900
Action
Library_start_date
repeat_library
Note
ID4657466146584662




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV15-19BGKcLV15-20
Sample_IDBGKcLV15-19BGKcLV15-20
Sample Name
Sample Name
RBPEIF2AEIF2A
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2020-09-122020-09-12
ProjectENCODE4ENCODE4
Note
ID53855386




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database