GSK3A      CRISPR in K562      Control: NT-BGKcLV13

General Information
RBPGSK3A
Cell_LineK562
MethodCRISPR
Exp_NameGSK3A-BGKcLV13-K562
ENCODE_series_IDENCSR883RLC
Batch_IDBGKcLV13
Pool IDPool-190423
Local_Set_Nameset42
ENCODE_KD_Exp_IDENCSR610HJO
ENCODE_CN_Exp_IDENCSR712CQC
Rep1GSK3A-BGKcLV13-41
Rep2GSK3A-BGKcLV13-42
CN1NT-BGKcLV13-1
CN2NT-BGKcLV13-2
Rep1_qPCR62.4
Rep2_qPCR62.9
Rep1_WB73.2
Rep2_WB71.9
Antibody Cat#MA3-038
Antibody Lot#RF227934
Antibody DCC IDENCAB957NOP
StatusReleased
ProjectENCODE4
ID175




Experiment Information (Status: NotSatisfied)
BGKcLV13-43BGKcLV13-44
idx488489
TRCN#_or_BGC#BGC#0000324BGC#0000324
shRNA_or_gRNA_sequenceCTCATTTGGGGTCGTGTACCCTCATTTGGGGTCGTGTACC
PAMaggagg
NameGSK3_93GSK3_93
Sample_IDBGKcLV13-43BGKcLV13-44
transduction_Date11/28/1811/28/18
daysDay 6Day 6
RBP_nameGSK3AGSK3A
qPCR_result32.944.2
Ave_qPCR38.6
RT-qPCR_primer-Fgagctgcagatcatgcgtaa
RT-qPCR_primer-Rgcacatattccagcaccaga
protein_conc46855469
WB_result13.65.3
Ave_WB9.5
WB_DONE_date1/4/19,2/15/19
MW51, 47KD
IPmouse
antibody_Cat#MA3-038
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
Action
Library_start_date
repeat_library
Note
ID45924593




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV13-43BGKcLV13-44
Sample_IDBGKcLV13-43BGKcLV13-44
Sample Name
Sample Name
RBPGSK3AGSK3A
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-08-102019-08-10
ProjectENCODE4ENCODE4
Note
ID29492950




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database