XRCC5      CRISPR in K562      Control: NT-BGKcLV11

General Information
RBPXRCC5
Cell_LineK562
MethodCRISPR
Exp_NameXRCC5-BGKcLV11-K562
ENCODE_series_IDENCSR678HMZ
Batch_IDBGKcLV11
Pool IDPool-190423
Local_Set_Nameset42
ENCODE_KD_Exp_IDENCSR276GMG
ENCODE_CN_Exp_IDENCSR292PXV
Rep1XRCC5-BGKcLV11-111
Rep2XRCC5-BGKcLV11-112
CN1NT-BGKcLV11-1
CN2NT-BGKcLV11-2
Rep1_qPCR12.4
Rep2_qPCR0.0
Rep1_WB69.5
Rep2_WB70.8
Antibody Cat#A302-627A
Antibody Lot#
Antibody DCC IDENCAB457QLP
StatusReleased
ProjectENCODE4
ID196




Experiment Information (Status: NotSatisfied)
BGKcLV11-107BGKcLV11-107BGKcLV11-107BGKcLV11-108BGKcLV11-108BGKcLV11-108
idx423425425424426426
TRCN#_or_BGC#BGC#0000083BGC#0000083BGC#0000083BGC#0000083BGC#0000083BGC#0000083
shRNA_or_gRNA_sequenceTGTTGTGCTGTGTATGGACGTGTTGTGCTGTGTATGGACGTGTTGTGCTGTGTATGGACGTGTTGTGCTGTGTATGGACGTGTTGTGCTGTGTATGGACGTGTTGTGCTGTGTATGGACG
PAMTGGTGGTGGTGGTGGTGG
NameXRCC5_80FXRCC5_80FXRCC5_80FXRCC5_80FXRCC5_80FXRCC5_80F
Sample_IDBGKcLV11-107BGKcLV11-107BGKcLV11-107BGKcLV11-108BGKcLV11-108BGKcLV11-108
transduction_Date8/3/188/3/188/3/188/3/188/3/188/3/18
daysDay 6Day 6Day 6Day 6Day 6Day 6
RBP_nameXRCC5XRCC5XRCC5XRCC5XRCC5XRCC5
qPCR_result49.749.749.734.134.134.1
Ave_qPCR41.941.941.9
RT-qPCR_primer-Fgcttggcaaagaaagatgagagcttggcaaagaaagatgagagcttggcaaagaaagatgaga
RT-qPCR_primer-Rgcttggcaaagaaagatgagagcttggcaaagaaagatgagagcttggcaaagaaagatgaga
protein_conc300130013001314431443144
WB_result39.056.068.449.561.566.1
Ave_WB44.258.8no needed
WB_DONE_date8/17/188/17/185/2/24
MW83 kDa83 kDa83 kDafu's
IP
antibody_Cat#A302-627A#2010042180Slot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM183.400000
Rep2_TPM19600000
Action
Library_start_date
repeat_library
Note
ID452545274529452645284530




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
XRCC5Product_ID: 2180S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: XRCC5-human		 CST_2180S_1_XRCC5.png<br>Caption: IP-WB analysis of 2180S whole cell lysate using the XRCC5 specific antibody, 2180S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the XRCC5-specific antibody, 2180S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 XRCC5-K562-CRISPR-2180S.png<br>Caption: Western blot following CRISPR against XRCC5 in K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against XRCC5. XRCC5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV11-107BGKcLV11-108
Sample_IDBGKcLV11-107BGKcLV11-108
Sample Name
Sample Name
RBPXRCC5XRCC5
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-08-102019-08-10
ProjectENCODE4ENCODE4
Note
ID27852786




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database