DGCR8      CRISPR in K562      Control: NT_BGKcLV02

General Information
RBPDGCR8
Cell_LineK562
MethodCRISPR
Exp_NameDGCR8-BGKcLV02-K562
ENCODE_series_ID
Batch_IDBGKcLV02
Pool IDPool-160201
Local_Set_NameBGKcLV02
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1DGCR8_BGKcLV02-19
Rep2DGCR8_BGKcLV02-20
CN1NT_BGKcLV02-1
CN2NT_BGKcLV02-2
Rep1_qPCR28.9
Rep2_qPCR14.4
Rep1_WB3.3
Rep2_WB0.0
Antibody Cat#A302-468A
Antibody Lot#A302-468A
Antibody DCC ID
StatusNotSatisfied
ProjectENCODE3
ID584




Experiment Information (Status: Released)
BGKcLV02-19-D41BGKcLV02-19-D41
idx4646
TRCN#_or_BGC#BGC#0000018BGC#0000018
shRNA_or_gRNA_sequenceCGCGACGTGCGGGCGGAGTGCGCGACGTGCGGGCGGAGTG
PAM
NameDGCR8-C1-ADGCR8-C1-A
Sample_IDBGKcLV02-19-D41BGKcLV02-19-D41
transduction_Date12/15/201512/15/2015
daysday41day41
RBP_nameDGCR8DGCR8
qPCR_result0.00.0
Ave_qPCR0.00.0
RT-qPCR_primer-Fccagccaatcagaagctcatccagccaatcagaagctcat
RT-qPCR_primer-Rtactcgtgcaggatgcagactactcgtgcaggatgcagac
protein_conc27482748
WB_result56.056.0
Ave_WB52.052.0
WB_DONE_date1/25/20161/25/2016
MW
IP
antibody_Cat#A302-468AA302-468A
Antibody DCC IDENCAB462ILE ENCAB462ILE
submitted_to_DCC_date9/28/169/28/16
Rep1_TPM40.240.2
Rep2_TPM38.438.4
Action
Library_start_date
repeat_library
Note
ID41444144




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DGCR8Product_ID: A302-468A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DGCR8-human		 HepG2_Bethyl_A302-468A_1_DGCR8.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').
 K562_Bethyl_A302-468A_1_DGCR8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').
 DGCR8-K562-D22-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 22 days after the CRISPR experiment process.
DGCR8-K562-D41-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 41 days after the CRISPR experiment process.
DGCR8-K562-D30-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 30 days after the CRISPR experiment process.
 AdrenalGland_Bethyl_A302-468A_1_DGCR8.png<br>Caption: IP-Western Blot analysis of human adrenal gland lysate using DGCR8 specific antibody. Lane 1 is 1% of 10 million tissue lysate input and lane 2 is 10% of IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8')). Lanes 3 and 4 indicate a technical replicate of lanes 1 and 2.




Experiment Status
BGKcLV02-19-D41BGKcLV02-19-D41
Sample_IDBGKcLV02-19-D41BGKcLV02-19-D41
Sample NameDGCR8-D41_BGKcLV02-19DGCR8-D41_BGKcLV02-19
Sample Name
RBPDGCR8DGCR8
Cell_LineK562K562
Exp UID
StatusReleasedReleased
Status_date2016-03-142016-03-14
ProjectENCODE3ENCODE3
Note
ID11451145




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database