RACK1      shRNA in K562      Batch: BGKLV21

Experiment Information (Status: NotSatisfied)
BGKLV21-13BGKLV21-14
idx284285
TRCN#_or_BGC#TRCN0000006472TRCN0000006472
shRNA_or_gRNA_sequenceGATGTGGTTATCTCCTCAGATGATGTGGTTATCTCCTCAGAT
PAM
NameGNB2L1GNB2L1
Sample_IDBGKLV21-13BGKLV21-14
transduction_Date10/9/201410/9/2014
days
RBP_nameGNB2L1GNB2L1
qPCR_result92.189.5
Ave_qPCR90.8
RT-qPCR_primer-Fatcgctactaccccgcagtt
RT-qPCR_primer-Ratccctggtcagtttccaca
protein_conc32862999
WB_result41.570.3
Ave_WB55.9
WB_DONE_date3/15/2015
MW
IP11
antibody_Cat#A302-545AA302-545A
Antibody DCC IDENCAB611SPGENCAB611SPG
submitted_to_DCC_date5/4/2015
Rep1_TPM1067.284580
Rep2_TPM1360.069510
Action
Library_start_date
repeat_library
Note
ID309310




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GNB2L1Product_ID: A302-545A
Lot_ID: 1
Source: Bethyl Labs
Target Name: GNB2L1-human		 HepG2_Bethyl_A302-545A_1_GNB2L1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GNB2L1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GNB2L1 antibody (lanes under 'GNB2L1').
 GNB2L1_Secondary_Western.png<br>Caption: Western blot following shRNA against GNB2L1 in K562 and HepG2 whole cell lysate using GNB2L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GNB2L1. Lanes 5-8 follow the same pattern, but in HepG2. GNB2L1 protein appears as the green band, Tubulin serves as a control and appears in red.
 GNB2L1_Secondary_Western.png<br>Caption: Western blot following shRNA against GNB2L1 in K562 and HepG2 whole cell lysate using GNB2L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GNB2L1. Lanes 5-8 follow the same pattern, but in HepG2. GNB2L1 protein appears as the green band, Tubulin serves as a control and appears in red.




Experiment Status
BGKLV21-13BGKLV21-14
Sample_IDBGKLV21-13BGKLV21-14
Sample Name
Sample Name
RBPGNB2L1GNB2L1
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-06-262019-06-26
ProjectENCODE3ENCODE3
Note
ID14631464




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database