FKBP4      shRNA in K562      Control: non-target_BGKLV21

General Information
RBPFKBP4
Cell_LineK562
MethodshRNA
Exp_NameFKBP4-BGKLV21-K562
ENCODE_series_IDENCSR355DBE
Batch_IDBGKLV21
Pool IDPool-150615
Local_Set_Nameset21
ENCODE_KD_Exp_IDENCSR379VXW
ENCODE_CN_Exp_IDENCSR572FFX
Rep1FKBP4_BGKLV21_11_17562
Rep2FKBP4_BGKLV21_12_17563
CN1non-target_BGKLV21_1
CN2non-target_BGKLV21_2
Rep1_qPCR92.9
Rep2_qPCR93.3
Rep1_WB83.3
Rep2_WB83.9
Antibody Cat#A301-427A
Antibody Lot#A301-427A
Antibody DCC IDENCAB145YUK
StatusReleased
ProjectENCODE3
ID508




Experiment Information (Status: Released)
BGKLV21-11BGKLV21-12
idx282283
TRCN#_or_BGC#TRCN0000152554TRCN0000152554
shRNA_or_gRNA_sequenceGCATGGAGAAAGGAGAACATTGCATGGAGAAAGGAGAACATT
PAM
NameFKBP4_54FKBP4_54
Sample_IDBGKLV21-11BGKLV21-12
transduction_Date10/9/201410/9/2014
days
RBP_nameFKBP4FKBP4
qPCR_result92.993.3
Ave_qPCR93.1
RT-qPCR_primer-Fctgacggaagaggaagatgg
RT-qPCR_primer-Ragtgcaacctccacgatagc
protein_conc32713340
WB_result83.383.9
Ave_WB83.6
WB_DONE_date3/19/2015
MW59kDa
IP11
antibody_Cat#A301-427AA301-427A
Antibody DCC IDENCAB145YUKENCAB145YUK
submitted_to_DCC_date4/2/2015
Rep1_TPM97.36044530
Rep2_TPM47.55915680
Action
Library_start_date5/19/20155/19/2015
repeat_library
Note
ID307308




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FKBP4Product_ID: A301-427A
Lot_ID: 1
Source: Bethyl Labs
Target Name: FKBP4-human
HepG2_Bethyl_A301-427A_1_FKBP4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FKBP4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FKBP4 antibody (lanes under 'FKBP4').
FKBP4_Secondary_Western.png<br>Caption: Western blot following shRNA against FKBP4 in K562 and HepG2 whole cell lysate using FKBP4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FKBP4. Lanes 5-7 follow the same pattern, but in HepG2. FKBP4 protein appears as the green band, GAPDH serves as a control and appears in red.
K562_BETHYL_A302427A_1_FKBP4.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FKBP4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FKBP4 antibody (lanes under 'FKBP4').
FKBP4_Secondary_Western.png<br>Caption: Western blot following shRNA against FKBP4 in K562 and HepG2 whole cell lysate using FKBP4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FKBP4. Lanes 5-7 follow the same pattern, but in HepG2. FKBP4 protein appears as the green band, GAPDH serves as a control and appears in red.




Experiment Status
BGKLV21-11BGKLV21-12
Sample_IDBGKLV21-11BGKLV21-12
Sample NameFKBP4_BGKLV21_11_17562FKBP4_BGKLV21_12_17563
Sample NameFKBP4_BGKLV21_11_17562FKBP4_BGKLV21_12_17563
RBPFKBP4FKBP4
Cell_LineK562K562
Exp UID
StatusReleasedReleased
Status_date2015-08-142015-08-14
ProjectENCODE3ENCODE3
Note
ID748749




Library-Prep Information
BGKLV21-11BGKLV21-12
Sample #
Sample NameFKBP4-BGKLV21-11FKBP4-BGKLV21-12
Sample_Name_AliasFKBP4_BGKLV21_11_17562FKBP4_BGKLV21_12_17563
Index Well PositionS07S08
Index_tableIDT_TruSeqSingleIndex_24IDT_TruSeqSingleIndex_24
LibPrep_date2015-05-192015-05-19
Lib_IDLib-150519Lib-150519
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_WellDNA_Library_LV21-24/set1/G5.pngDNA_Library_LV21-24/set1/H5.png
RIN9.99.9
libSample_RNA_WellRNA_Library_LV19-21-24/set2/C2.pngRNA_Library_LV19-21-24/set2/D2.png
SampleQC_method
SampleQC_date
Sample_IDBGKLV21-11BGKLV21-12
RBPFKBP4FKBP4
Batch_IDBGKLV21BGKLV21
WB_result83.30083.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCODE3ENCODE3
ID24342435




Sequencing Information
BGKLV21-11BGKLV21-12
Sample_IDBGKLV21-11BGKLV21-12
Sample NameFKBP4_BGKLV21_11_17562FKBP4_BGKLV21_12_17563
Pool IDPool-150615Pool-150615
LocalServer_folderset21set21
total_reads41,330,00042,260,000
total_aligned_reads35,751,82637,856,422
unique_aligned_reads32,616,64234,566,624
percent_uniqueAligned0.789180.81795
correlation_replicates0.9963210.996321
spikein_reads244,168217,334
percent_spikeins0.005910.00514
original_ReadLength100100
QC_StatusSubmittedSubmitted
ID10401041




Data Submission Information
BGKLV21-11BGKLV21-11
ENCODE_aliasbrenton-graveley:FKBP4_BGKLV21brenton-graveley:FKBP4-BGKLV21-K562
ENCODE_accessionENCSR379VXWENCSR355DBE
object_typeexperimentgene_silencing_series
assay_typeknockdown followed by RNA-seqknockdown followed by RNA-seq
Sample_IDBGKLV21-11BGKLV21-11
Sample NameFKBP4_BGKLV21_11_17562FKBP4_BGKLV21_11_17562
SelectedYesYes
StatusReleasedReleased
Status_Date2015-08-132022-03-01
ProjectENCODE3ENCODE3
protocol_URL
ID29606714




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
FKBP4_BGKLV21_11_17562_CAGATC_L001_R1_001.fastq.gzENCODE_DATA/set21/fastqfastqreadsknockdown followed by RNA-seqGraveley Lab1040HiSeq2000100paired-ended1non-target_BGKLV21_1_ATCACG_R1_001.fastq.gz,non-target_BGKLV21_2_CGATGT_R1_001.fastq.gzENCODE315924
FKBP4_BGKLV21_11_17562_CAGATC_L001_R2_001.fastq.gzENCODE_DATA/set21/fastqfastqreadsknockdown followed by RNA-seqGraveley Lab1040HiSeq2000100paired-ended2non-target_BGKLV21_1_ATCACG_R2_001.fastq.gz,non-target_BGKLV21_2_CGATGT_R2_001.fastq.gzENCODE315968
FKBP4_BGKLV21_11_17562_Aligned.sortedByCoord.out.bamENCODE_DATA/set21/STAR_ENCORE2_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562_CAGATC_L001_R1_001.fastq.gz,FKBP4_BGKLV21_11_17562_CAGATC_L001_R2_001.fastq.gzENCORE216012
FKBP4_BGKLV21_11_17562_Signal.Unique.strand+.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562/Aligned.sortedByCoord.out.bamENCORE216056
FKBP4_BGKLV21_11_17562_Signal.Unique.strand-.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562/Aligned.sortedByCoord.out.bamENCORE216100
FKBP4_BGKLV21_11_17562_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562/Aligned.sortedByCoord.out.bamENCORE216144
FKBP4_BGKLV21_11_17562_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562/Aligned.sortedByCoord.out.bamENCORE216188
FKBP4_BGKLV21_11_17562_quant.sfENCODE_DATA/set21/salmon_ENCORE2transcript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab1040GRCh38V40FKBP4_BGKLV21_11_17562_CAGATC_L001_R1_001.fastq.gz,FKBP4_BGKLV21_11_17562_CAGATC_L001_R2_001.fastq.gzENCORE216232
FKBP4_BGKLV21_12_17563_ACTTGA_L001_R1_001.fastq.gzENCODE_DATA/set21/fastqfastqreadsknockdown followed by RNA-seqGraveley Lab1041HiSeq2000100paired-ended1non-target_BGKLV21_1_ATCACG_R1_001.fastq.gz,non-target_BGKLV21_2_CGATGT_R1_001.fastq.gzENCODE315925
FKBP4_BGKLV21_12_17563_ACTTGA_L001_R2_001.fastq.gzENCODE_DATA/set21/fastqfastqreadsknockdown followed by RNA-seqGraveley Lab1041HiSeq2000100paired-ended2non-target_BGKLV21_1_ATCACG_R2_001.fastq.gz,non-target_BGKLV21_2_CGATGT_R2_001.fastq.gzENCODE315969
FKBP4_BGKLV21_12_17563_Aligned.sortedByCoord.out.bamENCODE_DATA/set21/STAR_ENCORE2_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563_ACTTGA_L001_R1_001.fastq.gz,FKBP4_BGKLV21_12_17563_ACTTGA_L001_R2_001.fastq.gzENCORE216013
FKBP4_BGKLV21_12_17563_Signal.Unique.strand+.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563/Aligned.sortedByCoord.out.bamENCORE216057
FKBP4_BGKLV21_12_17563_Signal.Unique.strand-.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563/Aligned.sortedByCoord.out.bamENCORE216101
FKBP4_BGKLV21_12_17563_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563/Aligned.sortedByCoord.out.bamENCORE216145
FKBP4_BGKLV21_12_17563_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set21/STAR_ENCORE2_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563/Aligned.sortedByCoord.out.bamENCORE216189
FKBP4_BGKLV21_12_17563_quant.sfENCODE_DATA/set21/salmon_ENCORE2transcript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab1041GRCh38V40FKBP4_BGKLV21_12_17563_ACTTGA_L001_R1_001.fastq.gz,FKBP4_BGKLV21_12_17563_ACTTGA_L001_R2_001.fastq.gzENCORE216233