RPS14 RNA-Seq in HepG2

General Information


RBP	RPS14
Cell_Line	HepG2
Method	CRISPR
Exp_Name	RPS14-BGHcLV50-HepG2
ENCODE_series_ID
Batch_ID	BGHcLV50
Pool ID	Pool-251003
Local_Set_Name	set80
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1	RPS14-BGHcLV50-97
Rep2	RPS14-BGHcLV50-98
CN1	NT-BGHcLV50-1
CN2	NT-BGHcLV50-2
Rep1_qPCR	50.1
Rep2_qPCR	64.6
Rep1_WB	44.8
Rep2_WB	57.2912
Antibody Cat#	A304-031A
Antibody Lot#	LOT #1
Antibody DCC ID
Status	Submitted
Project	ENCORE2
ID	1693

Experiment Information (Status: Submitting)

	BGHcLV50-97	BGHcLV50-98
idx	1920	1921
TRCN#_or_BGC#	BGC#0000811	BGC#0000811
shRNA_or_gRNA_sequence	GCCCGATCTTCATACCCGAG	GCCCGATCTTCATACCCGAG
PAM	CGG	CGG
Name	RPS14_70	RPS14_70
Sample_ID	BGHcLV50-97	BGHcLV50-98
transduction_Date	7/29/25	7/29/25
days	D6	D6
RBP_name	RPS14	RPS14
qPCR_result	50.1	64.6
Ave_qPCR	57.4
RT-qPCR_primer-F	gaccaagacccctggacct
RT-qPCR_primer-R	gagtgctgtcagaggggatg
protein_conc	1972	1966
WB_result	44.8	57.2912
Ave_WB
WB_DONE_date	9/2/25,8/28/25,8/26/
MW	16KD
IP
antibody_Cat#	A304-031A	LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	10109	10110

Western Blot Western Blot info was not avaliable

Experiment Status

	BGHcLV50-97	BGHcLV50-98
Sample_ID	BGHcLV50-97	BGHcLV50-98
Sample Name
Sample Name	RPS14-BGHcLV50-97	RPS14-BGHcLV50-98
RBP	RPS14	RPS14
Cell_Line	HepG2	HepG2
Exp UID
Status	Submitting	Submitting
Status_date	2026-10-30	2026-10-30
Project	ENCORE2	ENCORE2
Note
ID	14468	14469

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV50-97	BGHcLV50-98
Sample #	21	22
Sample Name	RPS14-BGHcLV50-97	RPS14-BGHcLV50-98
Sample_Name_Alias
Index Well Position	E12	F12
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-10-01	2025-10-01
Lib_ID	Lib-251001	Lib-251001
Tecan_Location
Tecan
Tecan_date
Size_bp	286	279
Peak_Molarity	81.90	76.90
libSampleQC_DNA_Well	DNA_Library_set80/set1/E12.png	DNA_Library_set80/set1/F12.png
RIN	9.6	9.6
libSample_RNA_Well	RNA_Library_set80/set1/E9.png	RNA_Library_set80/set1/F9.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-10-03	2025-10-03
Sample_ID	BGHcLV50-97	BGHcLV50-98
RBP	RPS14	RPS14
Batch_ID	BGHcLV50	BGHcLV50
WB_result	44.800	57.291
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4836	4837

Sequencing Information

	BGHcLV50-97	BGHcLV50-98
Sample_ID	BGHcLV50-97	BGHcLV50-98
Sample Name	RPS14-BGHcLV50-97	RPS14-BGHcLV50-98
Pool ID	Pool-251003	Pool-251003
LocalServer_folder	set80	set80
total_reads	15,042,754	17,209,206
total_aligned_reads	13,390,827	14,936,514
unique_aligned_reads	12,690,432	14,169,209
percent_uniqueAligned	0.84362	0.82335
correlation_replicates	0.991206	0.991206
spikein_reads	62,256	68,912
percent_spikeins	0.00414	0.00400
original_ReadLength	151	151
QC_Status	Submitting	Submitting
ID	3491	3492

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RPS14-BGHcLV50-97_S21_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set80/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3491	NovaSeq6000	100	paired-ended	1				NT-BGHcLV50-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32994
RPS14-BGHcLV50-97_S21_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set80/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3491	NovaSeq6000	100	paired-ended	2				NT-BGHcLV50-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33018
RPS14-BGHcLV50-97_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_S21_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS14-BGHcLV50-97_S21_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33042
RPS14-BGHcLV50-97_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_Aligned.sortedByCoord.out.bam		ENCORE2	33066
RPS14-BGHcLV50-97_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_Aligned.sortedByCoord.out.bam		ENCORE2	33090
RPS14-BGHcLV50-97_Signal.Unique.strand-.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_Aligned.sortedByCoord.out.bam		ENCORE2	33114
RPS14-BGHcLV50-97_Signal.Unique.strand+.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_Aligned.sortedByCoord.out.bam		ENCORE2	33138
RPS14-BGHcLV50-97_quant.sf	ENCODE_DATA/set80/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3491					GRCh38	V40	RPS14-BGHcLV50-97_S21_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS14-BGHcLV50-97_S21_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33162
RPS14-BGHcLV50-98_S22_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set80/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3492	NovaSeq6000	100	paired-ended	1				NT-BGHcLV50-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32995
RPS14-BGHcLV50-98_S22_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set80/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3492	NovaSeq6000	100	paired-ended	2				NT-BGHcLV50-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33019
RPS14-BGHcLV50-98_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_S22_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS14-BGHcLV50-98_S22_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33043
RPS14-BGHcLV50-98_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_Aligned.sortedByCoord.out.bam		ENCORE2	33067
RPS14-BGHcLV50-98_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_Aligned.sortedByCoord.out.bam		ENCORE2	33091
RPS14-BGHcLV50-98_Signal.Unique.strand-.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_Aligned.sortedByCoord.out.bam		ENCORE2	33115
RPS14-BGHcLV50-98_Signal.Unique.strand+.bw	ENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_Aligned.sortedByCoord.out.bam		ENCORE2	33139
RPS14-BGHcLV50-98_quant.sf	ENCODE_DATA/set80/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3492					GRCh38	V40	RPS14-BGHcLV50-98_S22_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS14-BGHcLV50-98_S22_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33163