non-target      CRISPR in HepG2      Batch: BGHcLV50

General Information

List KD experiments controlled by BGHcLV50-1 and BGHcLV50-2


NT-BGHcLV50-1,NT-BGHcLV50-2:No KD experiments controlled by NT-BGHcLV50-1 and NT-BGHcLV50-2 are available on the database




Experiment Information (Status: Sequenced)
BGHcLV50-1BGHcLV50-2
idx18181819
TRCN#_or_BGC#BGC#0000000BGC#0000000
shRNA_or_gRNA_sequenceCAGTCGGGCGTCATCATGATCAGTCGGGCGTCATCATGAT
PAM
Namenon-targetnon-target
Sample_IDBGHcLV50-1BGHcLV50-2
transduction_Date7/29/257/29/25
daysD6D6
RBP_namenon-targetnon-target
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc03005
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID1000710008




Western Blot
Western Blot info was not avaliable




Experiment Status
BGHcLV50-1BGHcLV50-2
Sample_IDBGHcLV50-1BGHcLV50-2
Sample Name
Sample NameNT-BGHcLV50-1NT-BGHcLV50-2
RBPnon-targetnon-target
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1444814449




Library-Prep Information
BGHcLV50-1BGHcLV50-2
Sample #12
Sample NameNT-BGHcLV50-1NT-BGHcLV50-2
Sample_Name_Alias
Index Well PositionA10B10
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-10-012025-10-01
Lib_IDLib-251001Lib-251001
Tecan_Location
Tecan
Tecan_date
Size_bp283291
Peak_Molarity37.2039.30
libSampleQC_DNA_WellDNA_Library_set80/set1/A10.pngDNA_Library_set80/set1/B10.png
RIN9.910.0
libSample_RNA_WellRNA_Library_set80/set1/A7.pngRNA_Library_set80/set1/B7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-10-032025-10-03
Sample_IDBGHcLV50-1BGHcLV50-2
RBPNTNT
Batch_IDBGHcLV50BGHcLV50
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48164817




Sequencing Information
BGHcLV50-1BGHcLV50-2
Sample_IDBGHcLV50-1BGHcLV50-2
Sample NameNT-BGHcLV50-1NT-BGHcLV50-2
Pool IDPool-251003Pool-251003
LocalServer_folderset80set80
total_reads24,258,39714,249,967
total_aligned_reads21,453,55512,579,323
unique_aligned_reads20,205,83511,869,445
percent_uniqueAligned0.832940.83295
correlation_replicates0.9922820.992282
spikein_reads65,79076,688
percent_spikeins0.002710.00538
original_ReadLength151151
QC_StatusReadyToSubmitReadyToSubmit
ID34713472




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
NT-BGHcLV50-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set80/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3471NovaSeq6000100paired-ended1ENCORE232986
NT-BGHcLV50-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set80/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3471NovaSeq6000100paired-ended2ENCORE233010
NT-BGHcLV50-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE233034
NT-BGHcLV50-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_Aligned.sortedByCoord.out.bamENCORE233058
NT-BGHcLV50-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_Aligned.sortedByCoord.out.bamENCORE233082
NT-BGHcLV50-1_Signal.Unique.strand-.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_Aligned.sortedByCoord.out.bamENCORE233106
NT-BGHcLV50-1_Signal.Unique.strand+.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_Aligned.sortedByCoord.out.bamENCORE233130
NT-BGHcLV50-1_quant.sfENCODE_DATA/set80/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3471GRCh38V40NT-BGHcLV50-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE233154
NT-BGHcLV50-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set80/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3472NovaSeq6000100paired-ended1ENCORE232987
NT-BGHcLV50-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set80/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3472NovaSeq6000100paired-ended2ENCORE233011
NT-BGHcLV50-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE233035
NT-BGHcLV50-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_Aligned.sortedByCoord.out.bamENCORE233059
NT-BGHcLV50-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_Aligned.sortedByCoord.out.bamENCORE233083
NT-BGHcLV50-2_Signal.Unique.strand-.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_Aligned.sortedByCoord.out.bamENCORE233107
NT-BGHcLV50-2_Signal.Unique.strand+.bwENCODE_DATA/set80/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_Aligned.sortedByCoord.out.bamENCORE233131
NT-BGHcLV50-2_quant.sfENCODE_DATA/set80/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3472GRCh38V40NT-BGHcLV50-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV50-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE233155