CCT3      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-9BGHcLV49-10
idx00
TRCN#_or_BGC#BGC#0000678BGC#0000678
shRNA_or_gRNA_sequenceGGTGATCAGTGCTTACCGCAGGTGATCAGTGCTTACCGCA
PAMAGGAGG
NameCCT3_94CCT3_94
Sample_IDBGHcLV49-9BGHcLV49-10
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCCT3CCT3
qPCR_result85.680.9
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc43605013
WB_result71.378.5
Ave_WB
WB_DONE_date6/17/25
MW61kd
IP
antibody_Cat#A303-459ALOT# 1
Antibody DCC IDENCAB809NWD
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98919892




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCT3Product_ID: A303-459A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCT3-human		 CCT3-hepg2-CRISPR-A303-459A-LICOR.png<br>Caption: Western blot following CRISPR against CCT3 in HepG2 whole cell lysate using CCT3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT3. CCT3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A303-459A_1_CCT3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCT3 specific antibody, A303-459A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCT3-specific antibody, A303-459A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCT3-K562-CRISPR-A303-459A.png<br>Caption: Western blot following CRISPR against CCT3 in K562 whole cell lysate using CCT3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT3. CCT3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-9BGHcLV49-10
Sample_IDBGHcLV49-9BGHcLV49-10
Sample Name
Sample NameCCT3-BGHcLV49-9CCT3-BGHcLV49-10
RBPCCT3CCT3
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1433014331




Library-Prep Information
BGHcLV49-9BGHcLV49-10
Sample #2728
Sample NameCCT3-BGHcLV49-9CCT3-BGHcLV49-10
Sample_Name_Alias
Index Well PositionE04F04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp260255
Peak_Molarity83.3090.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-9BGHcLV49-10
RBPCCT3CCT3
Batch_IDBGHcLV49BGHcLV49
WB_result71.30078.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47724773




Sequencing Information
BGHcLV49-9BGHcLV49-10
Sample_IDBGHcLV49-9BGHcLV49-10
Sample NameCCT3-BGHcLV49-9CCT3-BGHcLV49-10
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34993500




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database