RBM26      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-77BGHcLV49-78
idx00
TRCN#_or_BGC#BGC#0000760BGC#0000760
shRNA_or_gRNA_sequenceACCGGTACAATAGAAGACGAACCGGTACAATAGAAGACGA
PAMGGGGGG
NameRBM26_95RBM26_95
Sample_IDBGHcLV49-77BGHcLV49-78
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameRBM26RBM26
qPCR_result0.00.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc50453993
WB_result70.370.4
Ave_WB
WB_DONE_date7/3/25,7/10/25
MW114KD
IP
antibody_Cat#A301-216ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99619962




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM26Product_ID: A301-216A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM26-human		 RBM26-hepg2-CRISPR-A301-216A-LICOR.png<br>Caption: Western blot following CRISPR against RBM26 in HepG2 whole cell lysate using RBM26 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM26. RBM26 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-216A_1_RBM26.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM26 specific antibody, A301-216A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM26-specific antibody, A301-216A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM26-K562-CRISPR-A301-216A.png<br>Caption: Western blot following CRISPR against RBM26 in K562 whole cell lysate using RBM26 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM26. RBM26 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-77BGHcLV49-77BGHcLV49-78BGHcLV49-78
Sample_IDBGHcLV49-77BGHcLV49-77BGHcLV49-78BGHcLV49-78
Sample Name
Sample NameRBM26-BGHcLV49-77BRBM26-BGHcLV49-77BRBM26-BGHcLV49-78BRBM26-BGHcLV49-78B
RBPRBM26RBM26RBM26RBM26
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusSequencedSequencedSequencedSequenced
Status_date2025-10-302025-10-302025-10-302025-10-30
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14360144441436114445




Library-Prep Information
BGHcLV49-77BGHcLV49-77BGHcLV49-78BGHcLV49-78
Sample #57675868
Sample NameRBM26-BGHcLV49-77RBM26-BGHcLV49-77BRBM26-BGHcLV49-78RBM26-BGHcLV49-78B
Sample_Name_Alias
Index Well PositionC08E09D08F09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-09-112025-08-202025-09-11
Lib_IDLib-250820Lib-250911Lib-250820Lib-250911
Tecan_Location
Tecan
Tecan_date
Size_bp274284271290
Peak_Molarity68.1064.600.1279.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022TapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-152025-09-102025-09-15
Sample_IDBGHcLV49-77BGHcLV49-77BGHcLV49-78BGHcLV49-78
RBPRBM26RBM26RBM26RBM26
Batch_IDBGHcLV49BGHcLV49BGHcLV49BGHcLV49
WB_result70.30070.30070.40070.400
Library DescriptionTruSeq mRNATruSeq mRNATruSeq mRNATruSeq mRNA
Repeat_Library_SuffixBB
Lib_StatusNotToSequenceSendToSequenceNotToSequenceSendToSequence
ProjectENCORE2ENCORE2ENCORE2ENCORE2
ID4802481248034813




Sequencing Information
BGHcLV49-77BGHcLV49-78
Sample_IDBGHcLV49-77BGHcLV49-78
Sample NameRBM26-BGHcLV49-77BRBM26-BGHcLV49-78B
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35373538




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database