RBM26 RNA-Seq in HepG2

Experiment Information (Status: Sequenced)

	BGHcLV49-77	BGHcLV49-78
idx	1778	1779
TRCN#_or_BGC#	BGC#0000760	BGC#0000760
shRNA_or_gRNA_sequence	ACCGGTACAATAGAAGACGA	ACCGGTACAATAGAAGACGA
PAM	GGG	GGG
Name	RBM26_95	RBM26_95
Sample_ID	BGHcLV49-77	BGHcLV49-78
transduction_Date	6/6/25	6/6/25
days	D6	D6
RBP_name	RBM26	RBM26
qPCR_result	0.0	0.0
Ave_qPCR	0.0
RT-qPCR_primer-F	catgccaaagaaacggtgta
RT-qPCR_primer-R	aaggcagcataccaggaaga
protein_conc	5045	3993
WB_result	70.3	70.4
Ave_WB
WB_DONE_date	7/3/25,7/10/25
MW	114KD
IP
antibody_Cat#	A301-216A	LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9967	9968

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RBM26	Product_ID: A301-216A Lot_ID: 1 Source: Bethyl Labs Target Name: RBM26-human

Experiment Status

	BGHcLV49-77	BGHcLV49-77	BGHcLV49-78	BGHcLV49-78
Sample_ID	BGHcLV49-77	BGHcLV49-77	BGHcLV49-78	BGHcLV49-78
Sample Name
Sample Name	RBM26-BGHcLV49-77B	RBM26-BGHcLV49-77B	RBM26-BGHcLV49-78B	RBM26-BGHcLV49-78B
RBP	RBM26	RBM26	RBM26	RBM26
Cell_Line	HepG2	HepG2	HepG2	HepG2
Exp UID
Status	Sequenced	Sequenced	Sequenced	Sequenced
Status_date	2025-10-30	2025-10-30	2025-10-30	2025-10-30
Project	ENCORE2	ENCORE2	ENCORE2	ENCORE2
Note
ID	14360	14444	14361	14445

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV49-77	BGHcLV49-77	BGHcLV49-78	BGHcLV49-78
Sample #	57	67	58	68
Sample Name	RBM26-BGHcLV49-77	RBM26-BGHcLV49-77B	RBM26-BGHcLV49-78	RBM26-BGHcLV49-78B
Sample_Name_Alias
Index Well Position	C08	E09	D08	F09
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-08-20	2025-09-11	2025-08-20	2025-09-11
Lib_ID	Lib-250820	Lib-250911	Lib-250820	Lib-250911
Tecan_Location
Tecan
Tecan_date
Size_bp	274	284	271	290
Peak_Molarity	68.10	64.60	0.12	79.70
libSampleQC_DNA_Well		DNA_Library_set81/set2/E12.png		DNA_Library_set81/set2/F12.png
RIN		10.0		10.0
libSample_RNA_Well		RNA_Library_set81/set1/C8.png		RNA_Library_set81/set1/D8.png
SampleQC_method		TapeStation_2022		TapeStation_2022
SampleQC_date	2025-09-10	2025-09-15	2025-09-10	2025-09-15
Sample_ID	BGHcLV49-77	BGHcLV49-77	BGHcLV49-78	BGHcLV49-78
RBP	RBM26	RBM26	RBM26	RBM26
Batch_ID	BGHcLV49	BGHcLV49	BGHcLV49	BGHcLV49
WB_result	70.300	70.300	70.400	70.400
Library Description	TruSeq mRNA	TruSeq mRNA	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix		B		B
Lib_Status	NotToSequence	SendToSequence	NotToSequence	SendToSequence
Project	ENCORE2	ENCORE2	ENCORE2	ENCORE2
ID	4802	4812	4803	4813

Sequencing Information

	BGHcLV49-77	BGHcLV49-78
Sample_ID	BGHcLV49-77	BGHcLV49-78
Sample Name	RBM26-BGHcLV49-77B	RBM26-BGHcLV49-78B
Pool ID	Pool-250916	Pool-250916
LocalServer_folder	set81	set81
total_reads	26,956,601	26,496,798
total_aligned_reads	24,190,780	23,677,890
unique_aligned_reads	22,862,737	22,381,419
percent_uniqueAligned	0.84813	0.84468
correlation_replicates	0.999084	0.999084
spikein_reads	154,272	127,310
percent_spikeins	0.00572	0.00480
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3537	3538

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM26-BGHcLV49-77B_S152_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3537	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1B_S138_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2B_S144_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33202
RBM26-BGHcLV49-77B_S152_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3537	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1B_S138_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2B_S144_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33248
RBM26-BGHcLV49-77B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_S152_L008_R1_001.filtered.trimmed.paired.fastq.gz,RBM26-BGHcLV49-77B_S152_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33294
RBM26-BGHcLV49-77B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_Aligned.sortedByCoord.out.bam		ENCORE2	33340
RBM26-BGHcLV49-77B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_Aligned.sortedByCoord.out.bam		ENCORE2	33386
RBM26-BGHcLV49-77B_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_Aligned.sortedByCoord.out.bam		ENCORE2	33432
RBM26-BGHcLV49-77B_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_Aligned.sortedByCoord.out.bam		ENCORE2	33478
RBM26-BGHcLV49-77B_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3537					GRCh38	V40	RBM26-BGHcLV49-77B_S152_L008_R1_001.filtered.trimmed.paired.fastq.gz,RBM26-BGHcLV49-77B_S152_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33524
RBM26-BGHcLV49-78B_S150_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3538	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1B_S138_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2B_S144_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33203
RBM26-BGHcLV49-78B_S150_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3538	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1B_S138_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2B_S144_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33249
RBM26-BGHcLV49-78B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_S150_L008_R1_001.filtered.trimmed.paired.fastq.gz,RBM26-BGHcLV49-78B_S150_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33295
RBM26-BGHcLV49-78B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_Aligned.sortedByCoord.out.bam		ENCORE2	33341
RBM26-BGHcLV49-78B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_Aligned.sortedByCoord.out.bam		ENCORE2	33387
RBM26-BGHcLV49-78B_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_Aligned.sortedByCoord.out.bam		ENCORE2	33433
RBM26-BGHcLV49-78B_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_Aligned.sortedByCoord.out.bam		ENCORE2	33479
RBM26-BGHcLV49-78B_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3538					GRCh38	V40	RBM26-BGHcLV49-78B_S150_L008_R1_001.filtered.trimmed.paired.fastq.gz,RBM26-BGHcLV49-78B_S150_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33525