CCT2      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequencing)
BGHcLV49-7BGHcLV49-8
idx00
TRCN#_or_BGC#BGC#0000676BGC#0000676
shRNA_or_gRNA_sequenceCACAGACCATCATAGCGGGTCACAGACCATCATAGCGGGT
PAMTGGTGG
NameCCT2_92CCT2_92
Sample_IDBGHcLV49-7BGHcLV49-8
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCCT2CCT2
qPCR_result59.659.7
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc39995138
WB_result51.252.5
Ave_WB
WB_DONE_date6/17/25
MW57kd
IP
antibody_Cat#A303-478ALOT# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98879888




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCT2Product_ID: A303-479A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCT2-human		 CCT2-hepg2-CRISPR-A303-479A-LICOR.png<br>Caption: Western blot following CRISPR against CCT2 in HepG2 whole cell lysate using CCT2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT2. CCT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-7BGHcLV49-8
Sample_IDBGHcLV49-7BGHcLV49-8
Sample Name
Sample NameCCT2-BGHcLV49-7CCT2-BGHcLV49-8
RBPCCT2CCT2
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2025-09-292025-09-29
ProjectENCORE2ENCORE2
Note
ID1432814329




Library-Prep Information
BGHcLV49-7BGHcLV49-8
Sample #2526
Sample NameCCT2-BGHcLV49-7CCT2-BGHcLV49-8
Sample_Name_Alias
Index Well PositionC04D04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp268266
Peak_Molarity69.0087.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-7BGHcLV49-8
RBPCCT2CCT2
Batch_IDBGHcLV49BGHcLV49
WB_result51.20052.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47704771




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database