CCT2      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-7BGHcLV49-8
idx00
TRCN#_or_BGC#BGC#0000676BGC#0000676
shRNA_or_gRNA_sequenceCACAGACCATCATAGCGGGTCACAGACCATCATAGCGGGT
PAMTGGTGG
NameCCT2_92CCT2_92
Sample_IDBGHcLV49-7BGHcLV49-8
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCCT2CCT2
qPCR_result59.659.7
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc39995138
WB_result51.252.5
Ave_WB
WB_DONE_date6/17/25
MW57kd
IP
antibody_Cat#A303-478ALOT# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98899890




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCT2Product_ID: A303-479A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCT2-human		 CCT2-hepg2-CRISPR-A303-479A-LICOR.png<br>Caption: Western blot following CRISPR against CCT2 in HepG2 whole cell lysate using CCT2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT2. CCT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-7BGHcLV49-8
Sample_IDBGHcLV49-7BGHcLV49-8
Sample Name
Sample NameCCT2-BGHcLV49-7CCT2-BGHcLV49-8
RBPCCT2CCT2
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1432814329




Library-Prep Information
BGHcLV49-7BGHcLV49-8
Sample #2526
Sample NameCCT2-BGHcLV49-7CCT2-BGHcLV49-8
Sample_Name_Alias
Index Well PositionC04D04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp268266
Peak_Molarity69.0087.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-7BGHcLV49-8
RBPCCT2CCT2
Batch_IDBGHcLV49BGHcLV49
WB_result51.20052.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47704771




Sequencing Information
BGHcLV49-7BGHcLV49-8
Sample_IDBGHcLV49-7BGHcLV49-8
Sample NameCCT2-BGHcLV49-7CCT2-BGHcLV49-8
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34973498




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database