FTSJ3 RNA-Seq in HepG2

Experiment Information (Status: Sequenced)

	BGHcLV49-67	BGHcLV49-67	BGHcLV49-68	BGHcLV49-68
idx	1766	1768	1767	1769
TRCN#_or_BGC#	BGC#0001027	BGC#0001027	BGC#0001027	BGC#0001027
shRNA_or_gRNA_sequence	AGTGTTACGAACCATCCCCC	AGTGTTACGAACCATCCCCC	AGTGTTACGAACCATCCCCC	AGTGTTACGAACCATCCCCC
PAM	TGG	TGG	TGG	TGG
Name	FTSJ3_93	FTSJ3_93	FTSJ3_93	FTSJ3_93
Sample_ID	BGHcLV49-67	BGHcLV49-67	BGHcLV49-68	BGHcLV49-68
transduction_Date	6/6/25	6/6/25	6/6/25	6/6/25
days	D6	D6	D6	D6
RBP_name	FTSJ3	FTSJ3	FTSJ3	FTSJ3
qPCR_result	39.2		44.2
Ave_qPCR	41.7
RT-qPCR_primer-F	ccctgttgacttcctctcca
RT-qPCR_primer-R	tcagtggtagctggatgctg
protein_conc	3345	3345	3359	3359
WB_result	82.8		81.3
Ave_WB
WB_DONE_date	7/3/25	7/21/25		no band on the posio
MW	97kd	97kd
IP
antibody_Cat#	A304-199A	A304-198A	lot # 1	lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0	0	0
Rep2_TPM	0	0	0	0
Action	Ready		Ready
Library_start_date
repeat_library
Note
ID	9955	9957	9956	9958

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
FTSJ3	Product_ID: A304-199A Lot_ID: 1 Source: Bethyl Labs Target Name: FTSJ3-human

Experiment Status

	BGHcLV49-67	BGHcLV49-67	BGHcLV49-68	BGHcLV49-68
Sample_ID	BGHcLV49-67	BGHcLV49-67	BGHcLV49-68	BGHcLV49-68
Sample Name
Sample Name	FTSJ3-BGHcLV49-67		FTSJ3-BGHcLV49-68
RBP	FTSJ3	FTSJ3	FTSJ3	FTSJ3
Cell_Line	HepG2	HepG2	HepG2	HepG2
Exp UID
Status	Sequenced	NotSatisfied	Sequenced	NotSatisfied
Status_date	2025-10-30	2025-08-12	2025-10-30	2025-08-12
Project	ENCORE2	ENCORE2	ENCORE2	ENCORE2
Note
ID	14354	14410	14355	14411

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV49-67	BGHcLV49-68
Sample #	51	52
Sample Name	FTSJ3-BGHcLV49-67	FTSJ3-BGHcLV49-68
Sample_Name_Alias
Index Well Position	E07	F07
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-08-20	2025-08-20
Lib_ID	Lib-250820	Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp	262	255
Peak_Molarity	81.80	99.70
libSampleQC_DNA_Well	DNA_Library_set81/set1/E4.png	DNA_Library_set81/set1/F4.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set81/set1/E7.png	RNA_Library_set81/set1/F7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-09-10	2025-09-10
Sample_ID	BGHcLV49-67	BGHcLV49-68
RBP	FTSJ3	FTSJ3
Batch_ID	BGHcLV49	BGHcLV49
WB_result	82.800	81.300
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4796	4797

Sequencing Information

	BGHcLV49-67	BGHcLV49-68
Sample_ID	BGHcLV49-67	BGHcLV49-68
Sample Name	FTSJ3-BGHcLV49-67	FTSJ3-BGHcLV49-68
Pool ID	Pool-250916	Pool-250916
LocalServer_folder	set81	set81
total_reads	43,351,693	39,631,058
total_aligned_reads	35,513,397	32,709,813
unique_aligned_reads	33,431,488	30,794,458
percent_uniqueAligned	0.77117	0.77703
correlation_replicates	0.997228	0.997228
spikein_reads	78,680	72,564
percent_spikeins	0.00181	0.00183
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3523	3524

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
FTSJ3-BGHcLV49-67_S122_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3523	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1_S111_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33192
FTSJ3-BGHcLV49-67_S122_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3523	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1_S111_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33238
FTSJ3-BGHcLV49-67_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_S122_L008_R1_001.filtered.trimmed.paired.fastq.gz,FTSJ3-BGHcLV49-67_S122_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33284
FTSJ3-BGHcLV49-67_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_Aligned.sortedByCoord.out.bam		ENCORE2	33330
FTSJ3-BGHcLV49-67_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_Aligned.sortedByCoord.out.bam		ENCORE2	33376
FTSJ3-BGHcLV49-67_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_Aligned.sortedByCoord.out.bam		ENCORE2	33422
FTSJ3-BGHcLV49-67_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_Aligned.sortedByCoord.out.bam		ENCORE2	33468
FTSJ3-BGHcLV49-67_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3523					GRCh38	V40	FTSJ3-BGHcLV49-67_S122_L008_R1_001.filtered.trimmed.paired.fastq.gz,FTSJ3-BGHcLV49-67_S122_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33514
FTSJ3-BGHcLV49-68_S153_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3524	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1_S111_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33193
FTSJ3-BGHcLV49-68_S153_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3524	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1_S111_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33239
FTSJ3-BGHcLV49-68_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_S153_L008_R1_001.filtered.trimmed.paired.fastq.gz,FTSJ3-BGHcLV49-68_S153_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33285
FTSJ3-BGHcLV49-68_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_Aligned.sortedByCoord.out.bam		ENCORE2	33331
FTSJ3-BGHcLV49-68_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_Aligned.sortedByCoord.out.bam		ENCORE2	33377
FTSJ3-BGHcLV49-68_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_Aligned.sortedByCoord.out.bam		ENCORE2	33423
FTSJ3-BGHcLV49-68_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_Aligned.sortedByCoord.out.bam		ENCORE2	33469
FTSJ3-BGHcLV49-68_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3524					GRCh38	V40	FTSJ3-BGHcLV49-68_S153_L008_R1_001.filtered.trimmed.paired.fastq.gz,FTSJ3-BGHcLV49-68_S153_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33515