FTSJ3      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
idx0000
TRCN#_or_BGC#BGC#0001027BGC#0001027BGC#0001027BGC#0001027
shRNA_or_gRNA_sequenceAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCC
PAMTGGTGGTGGTGG
NameFTSJ3_93FTSJ3_93FTSJ3_93FTSJ3_93
Sample_IDBGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
transduction_Date6/6/256/6/256/6/256/6/25
daysD6D6D6D6
RBP_nameFTSJ3FTSJ3FTSJ3FTSJ3
qPCR_result39.244.2
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc3345334533593359
WB_result82.881.3
Ave_WB
WB_DONE_date7/3/257/21/25no band on the posio
MW97kd97kd
IP
antibody_Cat#A304-199AA304-198Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9949995199509952




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FTSJ3Product_ID: A304-199A
Lot_ID: 1
Source: Bethyl Labs
Target Name: FTSJ3-human		 FTSJ3-hepg2-CRISPR-A304-199A-LICOR.png<br>Caption: Western blot following CRISPR against FTSJ3 in HepG2 whole cell lysate using FTSJ3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FTSJ3. FTSJ3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 FTSJ3-K562-CRISPR-A304-199A.png<br>Caption: Western blot following CRISPR against FTSJ3 in K562 whole cell lysate using FTSJ3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FTSJ3. FTSJ3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
Sample_IDBGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
Sample Name
Sample NameFTSJ3-BGHcLV49-67FTSJ3-BGHcLV49-68
RBPFTSJ3FTSJ3FTSJ3FTSJ3
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusSequencedNotSatisfiedSequencedNotSatisfied
Status_date2025-10-302025-08-122025-10-302025-08-12
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14354144101435514411




Library-Prep Information
BGHcLV49-67BGHcLV49-68
Sample #5152
Sample NameFTSJ3-BGHcLV49-67FTSJ3-BGHcLV49-68
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp262255
Peak_Molarity81.8099.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-67BGHcLV49-68
RBPFTSJ3FTSJ3
Batch_IDBGHcLV49BGHcLV49
WB_result82.80081.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47964797




Sequencing Information
BGHcLV49-67BGHcLV49-68
Sample_IDBGHcLV49-67BGHcLV49-68
Sample NameFTSJ3-BGHcLV49-67FTSJ3-BGHcLV49-68
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35233524




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database