EZH2      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-61BGHcLV49-62
idx00
TRCN#_or_BGC#BGC#0001006BGC#0001006
shRNA_or_gRNA_sequenceTTATGATGGGAAAGTACACGTTATGATGGGAAAGTACACG
PAMGGGGGG
NameEZH2_81EZH2_81
Sample_IDBGHcLV49-61BGHcLV49-62
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEZH2EZH2
qPCR_result0.025.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc46221931
WB_result64.158.1
Ave_WB
WB_DONE_date7/3/25
MW85kd
IP
antibody_Cat#5246Slot # 10
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99419942




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EZH2Product_ID: 5246S
Lot_ID: 10
Source: Cell Signaling Technology
Target Name: EZH2-human		 EZH2-hepg2-CRISPR-5246S-LICOR.png<br>Caption: Western blot following CRISPR against EZH2 in HepG2 whole cell lysate using EZH2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EZH2. EZH2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5246S_10_EZH2.png<br>Caption: IP-WB analysis of 5246S whole cell lysate using the EZH2 specific antibody, 5246S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EZH2-specific antibody, 5246S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EZH2-K562-CRISPR-5246S.png<br>Caption: Western blot following CRISPR against EZH2 in K562 whole cell lysate using EZH2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EZH2. EZH2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-61BGHcLV49-62
Sample_IDBGHcLV49-61BGHcLV49-62
Sample Name
Sample Name
RBPEZH2EZH2
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1435214353




Library-Prep Information
BGHcLV49-61BGHcLV49-62
Sample #4950
Sample NameEZH2-BGHcLV49-61EZH2-BGHcLV49-62
Sample_Name_Alias
Index Well PositionC07D07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp274271
Peak_Molarity129.0080.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-61BGHcLV49-62
RBPEZH2EZH2
Batch_IDBGHcLV49BGHcLV49
WB_result64.10058.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID47944795




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database