EZH2 RNA-Seq in HepG2

Experiment Information (Status: Sequenced)

	BGHcLV49-61	BGHcLV49-62
idx	1760	1761
TRCN#_or_BGC#	BGC#0001006	BGC#0001006
shRNA_or_gRNA_sequence	TTATGATGGGAAAGTACACG	TTATGATGGGAAAGTACACG
PAM	GGG	GGG
Name	EZH2_81	EZH2_81
Sample_ID	BGHcLV49-61	BGHcLV49-62
transduction_Date	6/6/25	6/6/25
days	D6	D6
RBP_name	EZH2	EZH2
qPCR_result	0.0	25.0
Ave_qPCR	12.5
RT-qPCR_primer-F	tagggaagcagggactgaaa
RT-qPCR_primer-R	ttggtgtttgacaccgagaa
protein_conc	4622	1931
WB_result	64.1	58.1
Ave_WB
WB_DONE_date	7/3/25
MW	85kd
IP
antibody_Cat#	5246S	lot # 10
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9949	9950

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EZH2	Product_ID: 5246S Lot_ID: 10 Source: Cell Signaling Technology Target Name: EZH2-human

Experiment Status

	BGHcLV49-61	BGHcLV49-62
Sample_ID	BGHcLV49-61	BGHcLV49-62
Sample Name
Sample Name	EZH2-BGHcLV49-61	EZH2-BGHcLV49-62
RBP	EZH2	EZH2
Cell_Line	HepG2	HepG2
Exp UID
Status	Sequenced	Sequenced
Status_date	2025-10-30	2025-10-30
Project	ENCORE2	ENCORE2
Note
ID	14352	14353

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV49-61	BGHcLV49-62
Sample #	49	50
Sample Name	EZH2-BGHcLV49-61	EZH2-BGHcLV49-62
Sample_Name_Alias
Index Well Position	C07	D07
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-08-20	2025-08-20
Lib_ID	Lib-250820	Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp	274	271
Peak_Molarity	129.00	80.70
libSampleQC_DNA_Well	DNA_Library_set81/set1/C4.png	DNA_Library_set81/set1/D4.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set81/set1/C7.png	RNA_Library_set81/set1/D7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-09-10	2025-09-10
Sample_ID	BGHcLV49-61	BGHcLV49-62
RBP	EZH2	EZH2
Batch_ID	BGHcLV49	BGHcLV49
WB_result	64.100	58.100
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4794	4795

Sequencing Information

	BGHcLV49-61	BGHcLV49-62
Sample_ID	BGHcLV49-61	BGHcLV49-62
Sample Name	EZH2-BGHcLV49-61	EZH2-BGHcLV49-62
Pool ID	Pool-250916	Pool-250916
LocalServer_folder	set81	set81
total_reads	43,080,394	41,104,596
total_aligned_reads	37,101,448	36,061,104
unique_aligned_reads	34,975,853	34,022,098
percent_uniqueAligned	0.81187	0.82770
correlation_replicates	0.999001	0.999001
spikein_reads	92,264	91,700
percent_spikeins	0.00214	0.00223
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3521	3522

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
EZH2-BGHcLV49-61_S151_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3521	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1_S111_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33190
EZH2-BGHcLV49-61_S151_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3521	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1_S111_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33236
EZH2-BGHcLV49-61_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_S151_L008_R1_001.filtered.trimmed.paired.fastq.gz,EZH2-BGHcLV49-61_S151_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33282
EZH2-BGHcLV49-61_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_Aligned.sortedByCoord.out.bam		ENCORE2	33328
EZH2-BGHcLV49-61_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_Aligned.sortedByCoord.out.bam		ENCORE2	33374
EZH2-BGHcLV49-61_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_Aligned.sortedByCoord.out.bam		ENCORE2	33420
EZH2-BGHcLV49-61_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_Aligned.sortedByCoord.out.bam		ENCORE2	33466
EZH2-BGHcLV49-61_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3521					GRCh38	V40	EZH2-BGHcLV49-61_S151_L008_R1_001.filtered.trimmed.paired.fastq.gz,EZH2-BGHcLV49-61_S151_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33512
EZH2-BGHcLV49-62_S113_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3522	NovaSeq6000	100	paired-ended	1				NT-BGHcLV49-1_S111_L008_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33191
EZH2-BGHcLV49-62_S113_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set81/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3522	NovaSeq6000	100	paired-ended	2				NT-BGHcLV49-1_S111_L008_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV49-2_S136_L008_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	33237
EZH2-BGHcLV49-62_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_S113_L008_R1_001.filtered.trimmed.paired.fastq.gz,EZH2-BGHcLV49-62_S113_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33283
EZH2-BGHcLV49-62_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_Aligned.sortedByCoord.out.bam		ENCORE2	33329
EZH2-BGHcLV49-62_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_Aligned.sortedByCoord.out.bam		ENCORE2	33375
EZH2-BGHcLV49-62_Signal.Unique.strand-.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_Aligned.sortedByCoord.out.bam		ENCORE2	33421
EZH2-BGHcLV49-62_Signal.Unique.strand+.bw	ENCODE_DATA/set81/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_Aligned.sortedByCoord.out.bam		ENCORE2	33467
EZH2-BGHcLV49-62_quant.sf	ENCODE_DATA/set81/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3522					GRCh38	V40	EZH2-BGHcLV49-62_S113_L008_R1_001.filtered.trimmed.paired.fastq.gz,EZH2-BGHcLV49-62_S113_L008_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	33513