EZH2      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-61BGHcLV49-62
idx00
TRCN#_or_BGC#BGC#0001006BGC#0001006
shRNA_or_gRNA_sequenceTTATGATGGGAAAGTACACGTTATGATGGGAAAGTACACG
PAMGGGGGG
NameEZH2_81EZH2_81
Sample_IDBGHcLV49-61BGHcLV49-62
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEZH2EZH2
qPCR_result0.025.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc46221931
WB_result64.158.1
Ave_WB
WB_DONE_date7/3/25
MW85kd
IP
antibody_Cat#5246Slot # 10
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99439944




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EZH2Product_ID: 5246S
Lot_ID: 10
Source: Cell Signaling Technology
Target Name: EZH2-human		 EZH2-hepg2-CRISPR-5246S-LICOR.png<br>Caption: Western blot following CRISPR against EZH2 in HepG2 whole cell lysate using EZH2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EZH2. EZH2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5246S_10_EZH2.png<br>Caption: IP-WB analysis of 5246S whole cell lysate using the EZH2 specific antibody, 5246S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EZH2-specific antibody, 5246S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EZH2-K562-CRISPR-5246S.png<br>Caption: Western blot following CRISPR against EZH2 in K562 whole cell lysate using EZH2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EZH2. EZH2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-61BGHcLV49-62
Sample_IDBGHcLV49-61BGHcLV49-62
Sample Name
Sample NameEZH2-BGHcLV49-61EZH2-BGHcLV49-62
RBPEZH2EZH2
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1435214353




Library-Prep Information
BGHcLV49-61BGHcLV49-62
Sample #4950
Sample NameEZH2-BGHcLV49-61EZH2-BGHcLV49-62
Sample_Name_Alias
Index Well PositionC07D07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp274271
Peak_Molarity129.0080.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-61BGHcLV49-62
RBPEZH2EZH2
Batch_IDBGHcLV49BGHcLV49
WB_result64.10058.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47944795




Sequencing Information
BGHcLV49-61BGHcLV49-62
Sample_IDBGHcLV49-61BGHcLV49-62
Sample NameEZH2-BGHcLV49-61EZH2-BGHcLV49-62
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35213522




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database