EPRS      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-59BGHcLV49-60
idx00
TRCN#_or_BGC#BGC#0000990BGC#0000990
shRNA_or_gRNA_sequenceTCAGCATTTCCGTTGAAGAATCAGCATTTCCGTTGAAGAA
PAMGGGGGG
NameEPRS_81EPRS_81
Sample_IDBGHcLV49-59BGHcLV49-60
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEPRSEPRS
qPCR_result80.574.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc43724633
WB_result68.755.8
Ave_WB
WB_DONE_date6/24/25
MW171kd
IP
antibody_Cat#A303-958Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99419942




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EPRSProduct_ID: A303-958A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EPRS-human		 EPRS-hepg2-CRISPR-A303-958A-LICOR.png<br>Caption: Western blot following CRISPR against EPRS in HepG2 whole cell lysate using EPRS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EPRS. EPRS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EPRS-K562-CRISPR-A303-958A.png<br>Caption: Western blot following CRISPR against EPRS in K562 whole cell lysate using EPRS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EPRS. EPRS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-59BGHcLV49-60
Sample_IDBGHcLV49-59BGHcLV49-60
Sample Name
Sample NameEPRS-BGHcLV49-59EPRS-BGHcLV49-60
RBPEPRSEPRS
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1435014351




Library-Prep Information
BGHcLV49-59BGHcLV49-60
Sample #4748
Sample NameEPRS-BGHcLV49-59EPRS-BGHcLV49-60
Sample_Name_Alias
Index Well PositionA07B07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp264272
Peak_Molarity58.1093.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-59BGHcLV49-60
RBPEPRSEPRS
Batch_IDBGHcLV49BGHcLV49
WB_result68.70055.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47924793




Sequencing Information
BGHcLV49-59BGHcLV49-60
Sample_IDBGHcLV49-59BGHcLV49-60
Sample NameEPRS-BGHcLV49-59EPRS-BGHcLV49-60
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35193520




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database