EPRS      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-59BGHcLV49-60
idx00
TRCN#_or_BGC#BGC#0000990BGC#0000990
shRNA_or_gRNA_sequenceTCAGCATTTCCGTTGAAGAATCAGCATTTCCGTTGAAGAA
PAMGGGGGG
NameEPRS_81EPRS_81
Sample_IDBGHcLV49-59BGHcLV49-60
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEPRSEPRS
qPCR_result80.574.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc43724633
WB_result68.755.8
Ave_WB
WB_DONE_date6/24/25
MW171kd
IP
antibody_Cat#A303-958Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99399940




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EPRSProduct_ID: A303-958A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EPRS-human		 EPRS-hepg2-CRISPR-A303-958A-LICOR.png<br>Caption: Western blot following CRISPR against EPRS in HepG2 whole cell lysate using EPRS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EPRS. EPRS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EPRS-K562-CRISPR-A303-958A.png<br>Caption: Western blot following CRISPR against EPRS in K562 whole cell lysate using EPRS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EPRS. EPRS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-59BGHcLV49-60
Sample_IDBGHcLV49-59BGHcLV49-60
Sample Name
Sample Name
RBPEPRSEPRS
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1435014351




Library-Prep Information
BGHcLV49-59BGHcLV49-60
Sample #4748
Sample NameEPRS-BGHcLV49-59EPRS-BGHcLV49-60
Sample_Name_Alias
Index Well PositionA07B07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_method
SampleQC_date
Sample_IDBGHcLV49-59BGHcLV49-60
RBPEPRSEPRS
Batch_IDBGHcLV49BGHcLV49
WB_result68.70055.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusIndexAssignedIndexAssigned
ProjectENCORE2ENCORE2
ID47924793




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database