EIF2B5      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-53BGHcLV49-54
idx00
TRCN#_or_BGC#BGC#0000960BGC#0000960
shRNA_or_gRNA_sequenceGAAGCGGCGATCGAAGCTATGAAGCGGCGATCGAAGCTAT
PAMCGGCGG
NameEIF2B5_98EIF2B5_98
Sample_IDBGHcLV49-53BGHcLV49-54
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEIF2B5EIF2B5
qPCR_result65.461.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc35984458
WB_result59.776.6
Ave_WB
WB_DONE_date7/15/25
MW80KD
IP
antibody_Cat#A302-556Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99359936




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2B5Product_ID: A302-556A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF2B5-human		 EIF2B5-hepg2-CRISPR-A302-556A-LICOR.png<br>Caption: Western blot following CRISPR against EIF2B5 in HepG2 whole cell lysate using EIF2B5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B5. EIF2B5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EIF2B5-K562-CRISPR-A302-556A.png<br>Caption: Western blot following CRISPR against EIF2B5 in K562 whole cell lysate using EIF2B5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B5. EIF2B5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-53BGHcLV49-54
Sample_IDBGHcLV49-53BGHcLV49-54
Sample Name
Sample NameEIF2B5-BGHcLV49-53EIF2B5-BGHcLV49-54
RBPEIF2B5EIF2B5
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1434814349




Library-Prep Information
BGHcLV49-53BGHcLV49-54
Sample #4546
Sample NameEIF2B5-BGHcLV49-53EIF2B5-BGHcLV49-54
Sample_Name_Alias
Index Well PositionG06H06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp265257
Peak_Molarity72.2044.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-53BGHcLV49-54
RBPEIF2B5EIF2B5
Batch_IDBGHcLV49BGHcLV49
WB_result59.70076.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47904791




Sequencing Information
BGHcLV49-53BGHcLV49-54
Sample_IDBGHcLV49-53BGHcLV49-54
Sample NameEIF2B5-BGHcLV49-53EIF2B5-BGHcLV49-54
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35173518




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database