EFL1      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-49BGHcLV49-50
idx00
TRCN#_or_BGC#BGC#0000953BGC#0000953
shRNA_or_gRNA_sequenceTTAACAATAGACTTCAGCTATTAACAATAGACTTCAGCTA
PAMTGGTGG
NameEFTUD1_71EFTUD1_71
Sample_IDBGHcLV49-49BGHcLV49-50
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEFL1EFL1
qPCR_result63.663.2
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc48993845
WB_result82.692.9
Ave_WB
WB_DONE_date6/23/25
MW125KD
IP
antibody_Cat#A305-854A-Mlot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99299930




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EFL1Product_ID: A305-854A-M
Lot_ID: 1
Source: Bethyl Labs
Target Name: EFL1-human		 EFTUD1-hepg2-CRISPR-A305-854A-M-LICOR.png<br>Caption: Western blot following CRISPR against EFL1 in HepG2 whole cell lysate using EFL1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EFL1. EFL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EFL1-K562-CRISPR-A305-854A-M.png<br>Caption: Western blot following CRISPR against EFL1 in K562 whole cell lysate using EFL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EFL1. EFL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-49BGHcLV49-50
Sample_IDBGHcLV49-49BGHcLV49-50
Sample Name
Sample Name
RBPEFL1EFL1
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1434614347




Library-Prep Information
BGHcLV49-49BGHcLV49-50
Sample #4344
Sample NameEFL1-BGHcLV49-49EFL1-BGHcLV49-50
Sample_Name_Alias
Index Well PositionE06F06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp277266
Peak_Molarity90.0075.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-49BGHcLV49-50
RBPEFL1EFL1
Batch_IDBGHcLV49BGHcLV49
WB_result82.60092.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID47884789




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database