EEF2K      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-47BGHcLV49-48
idx00
TRCN#_or_BGC#BGC#0000952BGC#0000952
shRNA_or_gRNA_sequenceCAGAGAGGGTCCTTACTTGGCAGAGAGGGTCCTTACTTGG
PAMgggggg
NameEEF2K_82EEF2K_82
Sample_IDBGHcLV49-47BGHcLV49-48
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEEF2KEEF2K
qPCR_result42.039.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc34804959
WB_result67.263.2
Ave_WB
WB_DONE_date6/19/25
MW82KD
IP
antibody_Cat#A301-686Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99279928




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EEF2KProduct_ID: A301-686A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EEF2K-human		 EEF2K-hepg2-CRISPR-A301-686A-LICOR.png<br>Caption: Western blot following CRISPR against EEF2K in HepG2 whole cell lysate using EEF2K specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EEF2K. EEF2K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EEF2K-K562-CRISPR-A301-686A.png<br>Caption: Western blot following CRISPR against EEF2K in K562 whole cell lysate using EEF2K specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EEF2K. EEF2K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-47BGHcLV49-48
Sample_IDBGHcLV49-47BGHcLV49-48
Sample Name
Sample Name
RBPEEF2KEEF2K
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1434414345




Library-Prep Information
BGHcLV49-47BGHcLV49-48
Sample #4142
Sample NameEEF2K-BGHcLV49-47EEF2K-BGHcLV49-48
Sample_Name_Alias
Index Well PositionC06D06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp277275
Peak_Molarity84.4076.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-47BGHcLV49-48
RBPEEF2KEEF2K
Batch_IDBGHcLV49BGHcLV49
WB_result67.20063.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID47864787




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database