EEF2K      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-47BGHcLV49-48
idx00
TRCN#_or_BGC#BGC#0000952BGC#0000952
shRNA_or_gRNA_sequenceCAGAGAGGGTCCTTACTTGGCAGAGAGGGTCCTTACTTGG
PAMgggggg
NameEEF2K_82EEF2K_82
Sample_IDBGHcLV49-47BGHcLV49-48
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEEF2KEEF2K
qPCR_result42.039.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc34804959
WB_result67.263.2
Ave_WB
WB_DONE_date6/19/25
MW82KD
IP
antibody_Cat#A301-686Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99299930




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EEF2KProduct_ID: A301-686A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EEF2K-human		 EEF2K-hepg2-CRISPR-A301-686A-LICOR.png<br>Caption: Western blot following CRISPR against EEF2K in HepG2 whole cell lysate using EEF2K specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EEF2K. EEF2K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EEF2K-K562-CRISPR-A301-686A.png<br>Caption: Western blot following CRISPR against EEF2K in K562 whole cell lysate using EEF2K specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EEF2K. EEF2K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-47BGHcLV49-48
Sample_IDBGHcLV49-47BGHcLV49-48
Sample Name
Sample NameEEF2K-BGHcLV49-47EEF2K-BGHcLV49-48
RBPEEF2KEEF2K
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1434414345




Library-Prep Information
BGHcLV49-47BGHcLV49-48
Sample #4142
Sample NameEEF2K-BGHcLV49-47EEF2K-BGHcLV49-48
Sample_Name_Alias
Index Well PositionC06D06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp277275
Peak_Molarity84.4076.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-47BGHcLV49-48
RBPEEF2KEEF2K
Batch_IDBGHcLV49BGHcLV49
WB_result67.20063.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47864787




Sequencing Information
BGHcLV49-47BGHcLV49-48
Sample_IDBGHcLV49-47BGHcLV49-48
Sample NameEEF2K-BGHcLV49-47EEF2K-BGHcLV49-48
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35133514




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database