CWC15      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-19BGHcLV49-20
idx00
TRCN#_or_BGC#BGC#0000730BGC#0000730
shRNA_or_gRNA_sequenceGCAGGAATCTGGTCTAACCGGCAGGAATCTGGTCTAACCG
PAMTGGTGG
NameCWC15_97CWC15_97
Sample_IDBGHcLV49-19BGHcLV49-20
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCWC15CWC15
qPCR_result66.770.2
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc47914525
WB_result67.459.8
Ave_WB
WB_DONE_date6/17/25
MW27KD
IPRabbit / I
antibody_Cat#A304-900ALOT #1
Antibody DCC IDENCAB616CRG
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99019902




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CWC15Product_ID: A304-900A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CWC15-human		 CWC15-hepg2-CRISPR-A304-900A-LICOR.png<br>Caption: Western blot following CRISPR against CWC15 in HepG2 whole cell lysate using CWC15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CWC15. CWC15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A304-901A_1_CWC15.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CWC15 specific antibody, A304-900A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CWC15-specific antibody, A304-900A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 cwc15-K562-CRISPR-A304-900A.png<br>Caption: Western blot following CRISPR against CWC15 in K562 whole cell lysate using CWC15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CWC15. CWC15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-19BGHcLV49-20
Sample_IDBGHcLV49-19BGHcLV49-20
Sample Name
Sample NameCWC15-BGHcLV49-19CWC15-BGHcLV49-20
RBPCWC15CWC15
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1433814339




Library-Prep Information
BGHcLV49-19BGHcLV49-20
Sample #3536
Sample NameCWC15-BGHcLV49-19CWC15-BGHcLV49-20
Sample_Name_Alias
Index Well PositionE05F05
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp262252
Peak_Molarity89.4075.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-19BGHcLV49-20
RBPCWC15CWC15
Batch_IDBGHcLV49BGHcLV49
WB_result67.40059.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47804781




Sequencing Information
BGHcLV49-19BGHcLV49-20
Sample_IDBGHcLV49-19BGHcLV49-20
Sample NameCWC15-BGHcLV49-19CWC15-BGHcLV49-20
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35073508




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database