CCT4      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-11BGHcLV49-12
idx00
TRCN#_or_BGC#BGC#0000679BGC#0000679
shRNA_or_gRNA_sequenceTCGCAGCACTCGGTCCATCTTCGCAGCACTCGGTCCATCT
PAMGGGGGG
NameCCT4_86CCT4_86
Sample_IDBGHcLV49-11BGHcLV49-12
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCCT4CCT4
qPCR_result61.452.3
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc46653844
WB_result55.650.2
Ave_WB
WB_DONE_date6/17/25
MW58kd
IP
antibody_Cat#A304-725ALOT# 1
Antibody DCC IDENCAB174YJK
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98939894




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCT4Product_ID: A304-725A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCT4-human		 CCT4-hepg2-CRISPR-A304-725A-LICOR.png<br>Caption: Western blot following CRISPR against CCT4 in HepG2 whole cell lysate using CCT4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT4. CCT4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A304-725A_1_CCT4.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCT4 specific antibody, A304-725A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCT4-specific antibody, A304-725A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCT4-K562-CRISPR-A304-725A.png<br>Caption: Western blot following CRISPR against CCT4 in K562 whole cell lysate using CCT4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT4. CCT4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-11BGHcLV49-12
Sample_IDBGHcLV49-11BGHcLV49-12
Sample Name
Sample NameCCT4-BGHcLV49-11CCT4-BGHcLV49-12
RBPCCT4CCT4
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1433214333




Library-Prep Information
BGHcLV49-11BGHcLV49-12
Sample #2930
Sample NameCCT4-BGHcLV49-11CCT4-BGHcLV49-12
Sample_Name_Alias
Index Well PositionG04H04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp255258
Peak_Molarity82.4061.90
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-11BGHcLV49-12
RBPCCT4CCT4
Batch_IDBGHcLV49BGHcLV49
WB_result55.60050.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47744775




Sequencing Information
BGHcLV49-11BGHcLV49-12
Sample_IDBGHcLV49-11BGHcLV49-12
Sample NameCCT4-BGHcLV49-11CCT4-BGHcLV49-12
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35013502




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database