CCT4      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-11BGHcLV49-12
idx00
TRCN#_or_BGC#BGC#0000679BGC#0000679
shRNA_or_gRNA_sequenceTCGCAGCACTCGGTCCATCTTCGCAGCACTCGGTCCATCT
PAMGGGGGG
NameCCT4_86CCT4_86
Sample_IDBGHcLV49-11BGHcLV49-12
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCCT4CCT4
qPCR_result61.452.3
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc46653844
WB_result55.650.2
Ave_WB
WB_DONE_date6/17/25
MW58kd
IP
antibody_Cat#A304-725ALOT# 1
Antibody DCC IDENCAB174YJK
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98919892




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCT4Product_ID: A304-725A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCT4-human		 CCT4-hepg2-CRISPR-A304-725A-LICOR.png<br>Caption: Western blot following CRISPR against CCT4 in HepG2 whole cell lysate using CCT4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT4. CCT4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A304-725A_1_CCT4.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCT4 specific antibody, A304-725A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCT4-specific antibody, A304-725A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCT4-K562-CRISPR-A304-725A.png<br>Caption: Western blot following CRISPR against CCT4 in K562 whole cell lysate using CCT4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCT4. CCT4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-11BGHcLV49-12
Sample_IDBGHcLV49-11BGHcLV49-12
Sample Name
Sample Name
RBPCCT4CCT4
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1433214333




Library-Prep Information
BGHcLV49-11BGHcLV49-12
Sample #2930
Sample NameCCT4-BGHcLV49-11CCT4-BGHcLV49-12
Sample_Name_Alias
Index Well PositionG04H04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp255258
Peak_Molarity82.4061.90
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-11BGHcLV49-12
RBPCCT4CCT4
Batch_IDBGHcLV49BGHcLV49
WB_result55.60050.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID47744775




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database