SRA1      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-109BGHcLV49-110
idx00
TRCN#_or_BGC#BGC#0000887BGC#0000887
shRNA_or_gRNA_sequenceGCGCTCGCTGCTTACCAAGAGCGCTCGCTGCTTACCAAGA
PAMGGGGGG
NameSRA1_93SRA1_93
Sample_IDBGHcLV49-109BGHcLV49-110
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameSRA1SRA1
qPCR_result57.749.1
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc44704732
WB_result71.683.1
Ave_WB
WB_DONE_date7/3/25
MW26kd
IP
antibody_Cat#A300-743ALOT #1
Antibody DCC IDENCAB874LTS
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99959996




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRA1Product_ID: A300-743A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRA1-human		 SRA1-hepg2-CRISPR-A300-743A-LICOR.png<br>Caption: Western blot following CRISPR against SRA1 in HepG2 whole cell lysate using SRA1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRA1. SRA1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SRA1-K562-CRISPR-A300-743A.png<br>Caption: Western blot following CRISPR against SRA1 in K562 whole cell lysate using SRA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRA1. SRA1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-109BGHcLV49-110
Sample_IDBGHcLV49-109BGHcLV49-110
Sample Name
Sample Name
RBPSRA1SRA1
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1436814369




Library-Prep Information
BGHcLV49-109BGHcLV49-110
Sample #6566
Sample NameSRA1-BGHcLV49-109SRA1-BGHcLV49-110
Sample_Name_Alias
Index Well PositionC09D09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_method
SampleQC_date
Sample_IDBGHcLV49-109BGHcLV49-110
RBPSRA1SRA1
Batch_IDBGHcLV49BGHcLV49
WB_result71.60083.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusIndexAssignedIndexAssigned
ProjectENCORE2ENCORE2
ID48104811




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database