SRA1      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-109BGHcLV49-110
idx00
TRCN#_or_BGC#BGC#0000887BGC#0000887
shRNA_or_gRNA_sequenceGCGCTCGCTGCTTACCAAGAGCGCTCGCTGCTTACCAAGA
PAMGGGGGG
NameSRA1_93SRA1_93
Sample_IDBGHcLV49-109BGHcLV49-110
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameSRA1SRA1
qPCR_result57.749.1
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc44704732
WB_result71.683.1
Ave_WB
WB_DONE_date7/3/25
MW26kd
IP
antibody_Cat#A300-743ALOT #1
Antibody DCC IDENCAB874LTS
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99979998




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRA1Product_ID: A300-743A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRA1-human		 SRA1-hepg2-CRISPR-A300-743A-LICOR.png<br>Caption: Western blot following CRISPR against SRA1 in HepG2 whole cell lysate using SRA1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRA1. SRA1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SRA1-K562-CRISPR-A300-743A.png<br>Caption: Western blot following CRISPR against SRA1 in K562 whole cell lysate using SRA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRA1. SRA1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-109BGHcLV49-110
Sample_IDBGHcLV49-109BGHcLV49-110
Sample Name
Sample NameSRA1-BGHcLV49-109SRA1-BGHcLV49-110
RBPSRA1SRA1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1436814369




Library-Prep Information
BGHcLV49-109BGHcLV49-110
Sample #6566
Sample NameSRA1-BGHcLV49-109SRA1-BGHcLV49-110
Sample_Name_Alias
Index Well PositionC09D09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp260252
Peak_Molarity94.1076.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-109BGHcLV49-110
RBPSRA1SRA1
Batch_IDBGHcLV49BGHcLV49
WB_result71.60083.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48104811




Sequencing Information
BGHcLV49-109BGHcLV49-110
Sample_IDBGHcLV49-109BGHcLV49-110
Sample NameSRA1-BGHcLV49-109SRA1-BGHcLV49-110
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35353536




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database