RPS12      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-57BGHcLV47-58
idx00
TRCN#_or_BGC#BGC#0001262BGC#0001262
shRNA_or_gRNA_sequenceTCACCCTTCCTCGGCCATGGTCACCCTTCCTCGGCCATGG
PAMCGGCGG
NameRPS12_78URPS12_78U
Sample_IDBGHcLV47-57BGHcLV47-58
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameRPS12RPS12
qPCR_result51.742.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc30354260
WB_result89.493.8
Ave_WB
WB_DONE_date2/5/25
MW15KD
IP
antibody_Cat#A305-037ALOT #1
Antibody DCC IDENCAB450FMJ
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98449845




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS12Product_ID: A305-037A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS12-human		 RPS12-HEPG2-CRISPR-A305-037A.png<br>Caption: Western blot following CRISPR against RPS12 in HepG2 whole cell lysate using RPS12 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS12. RPS12 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A305-037A_1_RPS12.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS12 specific antibody, A305-037A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPS12-specific antibody, A305-037A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RPS12-K562-CRISPR-A305-037A.png<br>Caption: Western blot following CRISPR against RPS12 in K562 whole cell lysate using RPS12 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS12. RPS12 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-57BGHcLV47-58
Sample_IDBGHcLV47-57BGHcLV47-58
Sample Name
Sample NameRPS12-BGHcLV47-57RPS12-BGHcLV47-58
RBPRPS12RPS12
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1417214173




Library-Prep Information
BGHcLV47-57BGHcLV47-58
Sample #2526
Sample NameRPS12-BGHcLV47-57RPS12-BGHcLV47-58
Sample_Name_Alias
Index Well PositionA11B11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp262257
Peak_Molarity66.2021.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-57BGHcLV47-58
RBPRPS12RPS12
Batch_IDBGHcLV47BGHcLV47
WB_result89.40093.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47364737




Sequencing Information
BGHcLV47-57BGHcLV47-58
Sample_IDBGHcLV47-57BGHcLV47-58
Sample NameRPS12-BGHcLV47-57RPS12-BGHcLV47-58
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34393440




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database