MAPK1      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-43BGHcLV47-44
idx00
TRCN#_or_BGC#BGC#0001155BGC#0001155
shRNA_or_gRNA_sequenceTGGAGCCCTGTACCAACGTGTGGAGCCCTGTACCAACGTG
PAMTGGTGG
NameMAPK1_91MAPK1_91
Sample_IDBGHcLV47-43BGHcLV47-44
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameMAPK1MAPK1
qPCR_result68.372.4
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc42654905
WB_result66.261.3
Ave_WB
WB_DONE_date3/25/25
MW42,44kd
IP
antibody_Cat#4695Slot # 28
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98309831




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
MAPK1Product_ID: 4695S
Lot_ID: 28
Source: Cell Signaling Technology
Target Name: MAPK1-human		 MAPK1-HEPG2-CRISPR-4695S.png<br>Caption: Western blot following CRISPR against MAPK1 in HepG2 whole cell lysate using MAPK1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MAPK1. MAPK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_4695S_28_MAPK1.png<br>Caption: IP-WB analysis of 4695S whole cell lysate using the MAPK specific antibody, 4695S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the MAPK-specific antibody, 4695S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 MAPK1-K562-CRISPR-4695S.png<br>Caption: Western blot following CRISPR against MAPK1 in K562 whole cell lysate using MAPK1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MAPK1. MAPK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-43BGHcLV47-44
Sample_IDBGHcLV47-43BGHcLV47-44
Sample Name
Sample NameMAPK1-BGHcLV47-43MAPK1-BGHcLV47-44
RBPMAPK1MAPK1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1416414165




Library-Prep Information
BGHcLV47-43BGHcLV47-44
Sample #1718
Sample NameMAPK1-BGHcLV47-43MAPK1-BGHcLV47-44
Sample_Name_Alias
Index Well PositionA10B10
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp265266
Peak_Molarity83.3053.30
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-43BGHcLV47-44
RBPMAPK1MAPK1
Batch_IDBGHcLV47BGHcLV47
WB_result66.20061.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47284729




Sequencing Information
BGHcLV47-43BGHcLV47-44
Sample_IDBGHcLV47-43BGHcLV47-44
Sample NameMAPK1-BGHcLV47-43MAPK1-BGHcLV47-44
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34313432




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database