ASS1      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-3BGHcLV47-4
idx00
TRCN#_or_BGC#BGC#0001111BGC#0001111
shRNA_or_gRNA_sequenceCGTACTCCATCAGGTCATTGCGTACTCCATCAGGTCATTG
PAMCGGCGG
NameASS1_72ASS1_72
Sample_IDBGHcLV47-3BGHcLV47-4
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameASS1ASS1
qPCR_result58.948.8
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc30753826
WB_result63.267.3
Ave_WB
WB_DONE_date2/6/25
MW47kdTUBULIN
IP
antibody_Cat#70720Slot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID97909791




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ASS1Product_ID: 70720S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ASS1-human		 ASS1-HEPG2-CRISPR-70720S.png<br>Caption: Western blot following CRISPR against ASS1 in HepG2 whole cell lysate using ASS1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ASS1. ASS1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 CST_70720S_1_ASS1.png<br>Caption: IP-WB analysis of 70720S whole cell lysate using the ASS1 specific antibody, 70720S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ASS1-specific antibody, 70720S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ASS1-K562-CRISPR-70720S.png<br>Caption: Western blot following CRISPR against ASS1 in K562 whole cell lysate using ASS1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ASS1. ASS1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-3BGHcLV47-4
Sample_IDBGHcLV47-3BGHcLV47-4
Sample Name
Sample NameASS1-BGHcLV47-3ASS1-BGHcLV47-4
RBPASS1ASS1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1415014151




Library-Prep Information
BGHcLV47-3BGHcLV47-4
Sample #34
Sample NameASS1-BGHcLV47-3ASS1-BGHcLV47-4
Sample_Name_Alias
Index Well PositionC08D08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp267265
Peak_Molarity49.0049.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-3BGHcLV47-4
RBPASS1ASS1
Batch_IDBGHcLV47BGHcLV47
WB_result63.20067.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47144715




Sequencing Information
BGHcLV47-3BGHcLV47-4
Sample_IDBGHcLV47-3BGHcLV47-4
Sample NameASS1-BGHcLV47-3ASS1-BGHcLV47-4
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34173418




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database