ERAL1 RNA-Seq in HepG2

Experiment Information (Status: Sequenced)

	BGHcLV47-23	BGHcLV47-24
idx	1627	1628
TRCN#_or_BGC#	BGC#0000992	BGC#0000992
shRNA_or_gRNA_sequence	GGTGACTCCTTGCGTCCCCG	GGTGACTCCTTGCGTCCCCG
PAM	CGG	CGG
Name	ERAL1_88	ERAL1_88
Sample_ID	BGHcLV47-23	BGHcLV47-24
transduction_Date	1/28/25	1/28/25
days	D6	D6
RBP_name	ERAL1	ERAL1
qPCR_result	10.8	34.8
Ave_qPCR	22.8
RT-qPCR_primer-F	cctggagctctctttgttgg
RT-qPCR_primer-R	ggttccgtgtccacttgtct
protein_conc	2960	3581
WB_result	74.6	68.1
Ave_WB
WB_DONE_date	2/6/25
MW	48kd
IP		tubulin
antibody_Cat#	A305-092A	lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9816	9817

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
ERAL1	Product_ID: A305-092A Lot_ID: 1 Source: Bethyl Labs Target Name: ERAL1-human

Experiment Status

	BGHcLV47-23	BGHcLV47-24
Sample_ID	BGHcLV47-23	BGHcLV47-24
Sample Name
Sample Name	ERAL1-BGHcLV47-23	ERAL1-BGHcLV47-24
RBP	ERAL1	ERAL1
Cell_Line	HepG2	HepG2
Exp UID
Status	Sequenced	Sequenced
Status_date	2025-05-14	2025-05-14
Project	ENCORE2	ENCORE2
Note
ID	14158	14159

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV47-23	BGHcLV47-24
Sample #	11	12
Sample Name	ERAL1-BGHcLV47-23	ERAL1-BGHcLV47-24
Sample_Name_Alias
Index Well Position	C09	D09
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-04-23	2025-04-23
Lib_ID	Lib-250423	Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp	258	268
Peak_Molarity	58.30	56.40
libSampleQC_DNA_Well	DNA_Library_set78/set1/C7.png	DNA_Library_set78/set1/D7.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set78/set1/C2.png	RNA_Library_set78/set1/D2.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-04-22	2025-04-22
Sample_ID	BGHcLV47-23	BGHcLV47-24
RBP	ERAL1	ERAL1
Batch_ID	BGHcLV47	BGHcLV47
WB_result	74.600	68.100
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4722	4723

Sequencing Information

	BGHcLV47-23	BGHcLV47-24
Sample_ID	BGHcLV47-23	BGHcLV47-24
Sample Name	ERAL1-BGHcLV47-23	ERAL1-BGHcLV47-24
Pool ID	Pool-250422	Pool-250422
LocalServer_folder	set78	set78
total_reads	17,126,732	19,707,306
total_aligned_reads	14,449,079	17,600,972
unique_aligned_reads	13,566,716	16,564,609
percent_uniqueAligned	0.79214	0.84053
correlation_replicates	0.998424	0.998424
spikein_reads	17,628	18,594
percent_spikeins	0.00103	0.00094
original_ReadLength	101	101
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3425	3426

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
ERAL1-BGHcLV47-23_S52_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set78/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3425	NovaSeq6000	100	paired-ended	1				NT-BGHcLV47-1_S42_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV47-2_S43_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32556
ERAL1-BGHcLV47-23_S52_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set78/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3425	NovaSeq6000	100	paired-ended	2				NT-BGHcLV47-1_S42_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV47-2_S43_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32590
ERAL1-BGHcLV47-23_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_S52_L002_R1_001.filtered.trimmed.paired.fastq.gz,ERAL1-BGHcLV47-23_S52_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32624
ERAL1-BGHcLV47-23_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_Aligned.sortedByCoord.out.bam		ENCORE2	32658
ERAL1-BGHcLV47-23_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_Aligned.sortedByCoord.out.bam		ENCORE2	32692
ERAL1-BGHcLV47-23_Signal.Unique.strand-.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_Aligned.sortedByCoord.out.bam		ENCORE2	32726
ERAL1-BGHcLV47-23_Signal.Unique.strand+.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_Aligned.sortedByCoord.out.bam		ENCORE2	32760
ERAL1-BGHcLV47-23_quant.sf	ENCODE_DATA/set78/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3425					GRCh38	V40	ERAL1-BGHcLV47-23_S52_L002_R1_001.filtered.trimmed.paired.fastq.gz,ERAL1-BGHcLV47-23_S52_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32794
ERAL1-BGHcLV47-24_S53_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set78/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3426	NovaSeq6000	100	paired-ended	1				NT-BGHcLV47-1_S42_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV47-2_S43_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32557
ERAL1-BGHcLV47-24_S53_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set78/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3426	NovaSeq6000	100	paired-ended	2				NT-BGHcLV47-1_S42_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGHcLV47-2_S43_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32591
ERAL1-BGHcLV47-24_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_S53_L002_R1_001.filtered.trimmed.paired.fastq.gz,ERAL1-BGHcLV47-24_S53_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32625
ERAL1-BGHcLV47-24_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_Aligned.sortedByCoord.out.bam		ENCORE2	32659
ERAL1-BGHcLV47-24_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_Aligned.sortedByCoord.out.bam		ENCORE2	32693
ERAL1-BGHcLV47-24_Signal.Unique.strand-.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_Aligned.sortedByCoord.out.bam		ENCORE2	32727
ERAL1-BGHcLV47-24_Signal.Unique.strand+.bw	ENCODE_DATA/set78/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_Aligned.sortedByCoord.out.bam		ENCORE2	32761
ERAL1-BGHcLV47-24_quant.sf	ENCODE_DATA/set78/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3426					GRCh38	V40	ERAL1-BGHcLV47-24_S53_L002_R1_001.filtered.trimmed.paired.fastq.gz,ERAL1-BGHcLV47-24_S53_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32795