YARS      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-81BGHcLV45-82
idx00
TRCN#_or_BGC#BGC#0001217BGC#0001217
shRNA_or_gRNA_sequenceAGCCACATGTGGTTTGCCCGAGCCACATGTGGTTTGCCCG
PAMTGGTGG
NameYARS_92YARS_92
Sample_IDBGHcLV45-81BGHcLV45-82
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameYARSYARS
qPCR_result50.455.3
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc38833694
WB_result65.563.7
Ave_WB
WB_DONE_date10/25/24
MW59kd
IP
antibody_Cat#A305-064Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID77257726




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
YARSProduct_ID: A305-064A
Lot_ID: 1
Source: Bethyl Labs
Target Name: YARS-human		 YARS-HEPG2-CRISPR-A305-064A.png<br>Caption: Western blot following CRISPR against YARS in HepG2 whole cell lysate using YARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YARS. YARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 YARS-K562-CRISPR-A305-064A.png<br>Caption: Western blot following CRISPR against YARS in K562 whole cell lysate using YARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YARS. YARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-81BGHcLV45-82
Sample_IDBGHcLV45-81BGHcLV45-82
Sample Name
Sample NameYARS-BGHcLV45-81YARS-BGHcLV45-82
RBPYARSYARS
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1372413725




Library-Prep Information
BGHcLV45-81BGHcLV45-82
Sample #3940
Sample NameYARS-BGHcLV45-81YARS-BGHcLV45-82
Sample_Name_Alias
Index Well PositionG11H11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp291290
Peak_Molarity71.9058.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-81BGHcLV45-82
RBPYARSYARS
Batch_IDBGHcLV45BGHcLV45
WB_result65.50063.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46484649




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database