SYMPK      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-73BGHcLV45-74
idx12170
TRCN#_or_BGC#BGC#0001193BGC#0001193
shRNA_or_gRNA_sequenceTGTCATCGGCTTCATCGAGGTGTCATCGGCTTCATCGAGG
PAMAGGAGG
NameSYMPK_91BSYMPK_91B
Sample_IDBGHcLV45-73BGHcLV45-74
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameSYMPKSYMPK
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-FCGAAAATTTGTCATCGGCTT
RT-qPCR_primer-RTCACCACGTTCACATTCTCG
protein_conc44793448
WB_result67.773.7
Ave_WB
WB_DONE_date10/15/24
MW141kd
IP
antibody_Cat#13071Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID77157716




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SYMPKProduct_ID: 13071S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SYMPK-human		 SYMPK-HEPG2-CRISPR-13071S.png<br>Caption: Western blot following CRISPR against SYMPK in HepG2 whole cell lysate using SYMPK specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SYMPK. SYMPK protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13071S_1_SYMPK.png<br>Caption: IP-WB analysis of 13071S whole cell lysate using the SYMPK specific antibody, 13071S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SYMPK-specific antibody, 13071S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.




Experiment Status
BGHcLV45-73BGHcLV45-74
Sample_IDBGHcLV45-73BGHcLV45-74
Sample Name
Sample NameSYMPK-BGHcLV45-73SYMPK-BGHcLV45-74
RBPSYMPKSYMPK
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1372013721




Library-Prep Information
BGHcLV45-73BGHcLV45-74
Sample #3536
Sample NameSYMPK-BGHcLV45-73SYMPK-BGHcLV45-74
Sample_Name_Alias
Index Well PositionC11D11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp296292
Peak_Molarity88.6080.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-73BGHcLV45-74
RBPSYMPKSYMPK
Batch_IDBGHcLV45BGHcLV45
WB_result67.70073.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46444645




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database