SENP3      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-61BGHcLV45-62
idx12171217
TRCN#_or_BGC#BGC#0001187BGC#0001187
shRNA_or_gRNA_sequenceCCCCGACTCAAGTCAGGTGGCCCCGACTCAAGTCAGGTGG
PAMAGGAGG
NameSENP3_85SENP3_85
Sample_IDBGHcLV45-61BGHcLV45-62
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameSENP3SENP3
qPCR_result45.535.9
Ave_qPCR40.7
RT-qPCR_primer-Fggagacctggtcatggacac
RT-qPCR_primer-Raccccatcataacccttggt
protein_conc34893183
WB_result91.084.6
Ave_WB
WB_DONE_date10/18/24
MW75kd
IP
antibody_Cat#5591Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID77037704




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SENP3Product_ID: 5591S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SENP3-human		 SENP3-HEPG2-CRISPR-5591S.png<br>Caption: Western blot following CRISPR against SENP3 in HepG2 whole cell lysate using SENP3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SENP3. SENP3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5591S_1_SENP3.png<br>Caption: IP-WB analysis of 5591S whole cell lysate using the SENP3 specific antibody, 5591S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SENP3-specific antibody, 5591S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SENP3-K562-CRISPR-5591S.png<br>Caption: Western blot following CRISPR against SENP3 in K562 whole cell lysate using SENP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SENP3. SENP3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-61BGHcLV45-62
Sample_IDBGHcLV45-61BGHcLV45-62
Sample Name
Sample NameSENP3-BGHcLV45-61SENP3-BGHcLV45-62
RBPSENP3SENP3
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1371213713




Library-Prep Information
BGHcLV45-61BGHcLV45-62
Sample #2728
Sample NameSENP3-BGHcLV45-61SENP3-BGHcLV45-62
Sample_Name_Alias
Index Well PositionC10D10
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp286296
Peak_Molarity76.1077.60
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-61BGHcLV45-62
RBPSENP3SENP3
Batch_IDBGHcLV45BGHcLV45
WB_result91.00084.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46364637




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database