ALYREF      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-5BGHcLV45-6
idx14381439
TRCN#_or_BGC#BGC#0001108BGC#0001108
shRNA_or_gRNA_sequenceGCCGCGCGAGTGAATCGAGGGCCGCGCGAGTGAATCGAGG
PAMCGGCGG
NameALYREF_97ALYREF_97
Sample_IDBGHcLV45-5BGHcLV45-6
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameALYREFALYREF
qPCR_result2.527.1
Ave_qPCR14.8
RT-qPCR_primer-FCTGAAGGCCATGAAGCAGTA
RT-qPCR_primer-RTCTGTGACGTGACAAGCTGA
protein_conc37863221
WB_result54.262.5
Ave_WB
WB_DONE_date10/15/24
MW27KD
IP
antibody_Cat#12655Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76457646




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ALYREFProduct_ID: 12655S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ALYREF-human		 ALYREF-HEPG2-CRISPR-12655S.png<br>Caption: Western blot following CRISPR against ALYREF in HepG2 whole cell lysate using ALYREF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ALYREF. ALYREF protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12655S_1_ALYREF.png<br>Caption: IP-WB analysis of 12655S whole cell lysate using the ALYREF specific antibody, 12655S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ALYREF-specific antibody, 12655S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ALYREF-K562-CRISPR-12655S.png<br>Caption: Western blot following CRISPR against ALYREF in K562 whole cell lysate using ALYREF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ALYREF. ALYREF protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-5BGHcLV45-6
Sample_IDBGHcLV45-5BGHcLV45-6
Sample Name
Sample NameALYREF-BGHcLV45-5ALYREF-BGHcLV45-6
RBPALYREFALYREF
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1369013691




Library-Prep Information
BGHcLV45-5BGHcLV45-6
Sample #56
Sample NameALYREF-BGHcLV45-5ALYREF-BGHcLV45-6
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp275280
Peak_Molarity91.8075.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-5BGHcLV45-6
RBPALYREFALYREF
Batch_IDBGHcLV45BGHcLV45
WB_result54.20062.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46144615




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database