ADD1      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-3BGHcLV45-4
idx14361437
TRCN#_or_BGC#BGC#0001107BGC#0001107
shRNA_or_gRNA_sequenceGATCCTCTAAGATCGTTCACGATCCTCTAAGATCGTTCAC
PAMAGGAGG
NameADD1_97ADD1_97
Sample_IDBGHcLV45-3BGHcLV45-4
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameADD1ADD1
qPCR_result71.566.0
Ave_qPCR68.7
RT-qPCR_primer-Ftctttgggtggtctcagctt
RT-qPCR_primer-Ragaagcccaaaagggacaat
protein_conc50634978
WB_result70.560.3
Ave_WB
WB_DONE_date10/9/24
MW120KD
IP
antibody_Cat#70174Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76437644




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ADD1Product_ID: 70174S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ADD1-human		 ADD1-HEPG2-CRISPR-70174S.png<br>Caption: Western blot following CRISPR against ADD1 in HepG2 whole cell lysate using ADD1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADD1. ADD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_70174S_1_ADD1.png<br>Caption: IP-WB analysis of 70174S whole cell lysate using the ADD1 specific antibody, 70174S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ADD1-specific antibody, 70174S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ADD1-K562-CRISPR-70174S.png<br>Caption: Western blot following CRISPR against ADD1 in K562 whole cell lysate using ADD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADD1. ADD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-3BGHcLV45-4
Sample_IDBGHcLV45-3BGHcLV45-4
Sample Name
Sample NameADD1-BGHcLV45-3ADD1-BGHcLV45-4
RBPADD1ADD1
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1368813689




Library-Prep Information
BGHcLV45-3BGHcLV45-4
Sample #34
Sample NameADD1-BGHcLV45-3ADD1-BGHcLV45-4
Sample_Name_Alias
Index Well PositionC07D07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp280283
Peak_Molarity77.8081.30
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-3BGHcLV45-4
RBPADD1ADD1
Batch_IDBGHcLV45BGHcLV45
WB_result70.50060.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46124613




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database