DEK      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-19BGHcLV45-20
idx12171217
TRCN#_or_BGC#BGC#0000310BGC#0000310
shRNA_or_gRNA_sequenceGTTCTTTCTCGGACGCGGGCGTTCTTTCTCGGACGCGGGC
PAMtggtgg
NameDEK_97DEK_97
Sample_IDBGHcLV45-19BGHcLV45-20
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameDEKDEK
qPCR_result35.639.7
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc36093812
WB_result72.880.8
Ave_WB
WB_DONE_date10/10/24
MW43kd
IP
antibody_Cat#720215SD249202
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76597660




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DEKProduct_ID: 720215
Lot_ID: SD249202
Source: Thermo Fisher
Target Name: DEK-human		 DEK-HEPG2-CRISPR-720215.png<br>Caption: Western blot following CRISPR against DEK in HepG2 whole cell lysate using DEK specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DEK. DEK protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_720215_SD249202_DEK.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DEK specific antibody, 720215. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DEK-specific antibody, 720215. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DEK-K562-CRISPR-720215.png<br>Caption: Western blot following CRISPR against DEK in K562 whole cell lysate using DEK specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DEK. DEK protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-19BGHcLV45-20
Sample_IDBGHcLV45-19BGHcLV45-20
Sample Name
Sample NameDEK-BGHcLV45-19DEK-BGHcLV45-20
RBPDEKDEK
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1370013701




Library-Prep Information
BGHcLV45-19BGHcLV45-20
Sample #1516
Sample NameDEK-BGHcLV45-19DEK-BGHcLV45-20
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp291294
Peak_Molarity65.2057.90
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-19BGHcLV45-20
RBPDEKDEK
Batch_IDBGHcLV45BGHcLV45
WB_result72.80080.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46244625




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database