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TARS RNA-Seq in HepG2



TARS      CRISPR in HepG2      Control:

General Information
RBPTARS
Cell_LineHepG2
MethodCRISPR
Exp_NameTARS-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1TARS-BGHcLV41-93
Rep2TARS-BGHcLV41-94
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR57.7
Rep2_qPCR66.2
Rep1_WB85.5
Rep2_WB81.7
Antibody Cat#A304-755A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1465




Experiment Information (Status: Submitting)
BGHcLV41-93BGHcLV41-94
idx13661367
TRCN#_or_BGC#BGC#0001066BGC#0001066
shRNA_or_gRNA_sequenceTCAGAATTCCTCCACGTACTTCAGAATTCCTCCACGTACT
PAMGGGGGG
NameTARS_90TARS_90
Sample_IDBGHcLV41-93BGHcLV41-94
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameTARSTARS
qPCR_result57.766.2
Ave_qPCR62.0
RT-qPCR_primer-Ftggatccaggctgtacattg
RT-qPCR_primer-Rcccgtggaccttattgtctc
protein_conc33963352
WB_result85.581.7
Ave_WB83.6
WB_DONE_date1/29/24
MW83kd
IP
antibody_Cat#A304-755Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75637564




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
TARSProduct_ID: A304-755A
Lot_ID: 1
Source: Bethyl Labs
Target Name: TARS-human		 TARS-HEPG2-CRISPR-A304-755A.png<br>Caption: Western blot following CRISPR against TARS in HepG2 whole cell lysate using TARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against TARS. TARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 TARS-K562-CRISPR-A304-755A.png<br>Caption: Western blot following CRISPR against TARS in K562 whole cell lysate using TARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against TARS. TARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-93BGHcLV41-94
Sample_IDBGHcLV41-93BGHcLV41-94
Sample Name
Sample NameTARS-BGHcLV41-93TARS-BGHcLV41-94
RBPTARSTARS
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1299012991




Library-Prep Information
BGHcLV41-93BGHcLV41-94
Sample #4344
Sample NameTARS-BGHcLV41-93TARS-BGHcLV41-94
Sample_Name_Alias
Index Well PositionC06D06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp293291
Peak_Molarity90.9084.40
libSampleQC_DNA_WellDNA_Library_set70/set1/C6.pngDNA_Library_set70/set1/D6.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set70/set1/G11.pngRNA_Library_set70/set1/H11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-93BGHcLV41-94
RBPTARSTARS
Batch_IDBGHcLV41BGHcLV41
WB_result85.50081.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42684269




Sequencing Information
BGHcLV41-93BGHcLV41-94
Sample_IDBGHcLV41-93BGHcLV41-94
Sample NameTARS-BGHcLV41-93TARS-BGHcLV41-94
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads37,316,23037,765,909
total_aligned_reads34,781,51835,592,021
unique_aligned_reads32,907,74233,685,306
percent_uniqueAligned0.881860.89195
correlation_replicates0.9992550.999255
spikein_reads3,268792
percent_spikeins0.000090.00002
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29872988




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database