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SRP72 RNA-Seq in HepG2



SRP72      CRISPR in HepG2      Control:

General Information
RBPSRP72
Cell_LineHepG2
MethodCRISPR
Exp_NameSRP72-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SRP72-BGHcLV41-91
Rep2SRP72-BGHcLV41-92
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR61.1
Rep2_qPCR49.5
Rep1_WB84.5
Rep2_WB87.5
Antibody Cat#A304-594A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1464




Experiment Information (Status: Submitting)
BGHcLV41-91BGHcLV41-92
idx13641365
TRCN#_or_BGC#BGC#0001050BGC#0001050
shRNA_or_gRNA_sequenceGGGTGTCAGTACCTGCGCTGGGGTGTCAGTACCTGCGCTG
PAMTGGTGG
NameSRP72_82SRP72_82
Sample_IDBGHcLV41-91BGHcLV41-92
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameSRP72SRP72
qPCR_result61.149.5
Ave_qPCR55.3
RT-qPCR_primer-Ftcaaatatgggcggaagaag
RT-qPCR_primer-Raataagctgtgccagggtgt
protein_conc34953771
WB_result84.587.5
Ave_WB86.0
WB_DONE_date1/29/24
MW75kd
IP
antibody_Cat#A304-594Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75617562




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRP72Product_ID: A304-594A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRP72-human		 SRP72-HEPG2-CRISPR-A304-594A.png<br>Caption: Western blot following CRISPR against SRP72 in HepG2 whole cell lysate using SRP72 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP72. SRP72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SRP72-K562-CRISPR-A304-594A.png<br>Caption: Western blot following CRISPR against SRP72 in K562 whole cell lysate using SRP72 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP72. SRP72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-91BGHcLV41-92
Sample_IDBGHcLV41-91BGHcLV41-92
Sample Name
Sample NameSRP72-BGHcLV41-91SRP72-BGHcLV41-92
RBPSRP72SRP72
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1298812989




Library-Prep Information
BGHcLV41-91BGHcLV41-92
Sample #4142
Sample NameSRP72-BGHcLV41-91SRP72-BGHcLV41-92
Sample_Name_Alias
Index Well PositionA06B06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp313292
Peak_Molarity97.0076.90
libSampleQC_DNA_WellDNA_Library_set70/set1/A6.pngDNA_Library_set70/set1/B6.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set70/set1/E11.pngRNA_Library_set70/set1/F11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-91BGHcLV41-92
RBPSRP72SRP72
Batch_IDBGHcLV41BGHcLV41
WB_result84.50087.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42664267




Sequencing Information
BGHcLV41-91BGHcLV41-92
Sample_IDBGHcLV41-91BGHcLV41-92
Sample NameSRP72-BGHcLV41-91SRP72-BGHcLV41-92
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads46,846,90933,099,905
total_aligned_reads43,539,72730,694,642
unique_aligned_reads41,216,54429,050,289
percent_uniqueAligned0.879810.87765
correlation_replicates0.9988230.998823
spikein_reads330986
percent_spikeins0.000010.00003
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29852986




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database