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MARS RNA-Seq in HepG2



MARS      CRISPR in HepG2      Control:

General Information
RBPMARS
Cell_LineHepG2
MethodCRISPR
Exp_NameMARS-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1MARS-BGHcLV41-85
Rep2MARS-BGHcLV41-86
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR32.6
Rep2_qPCR26.4
Rep1_WB
Rep2_WB
Antibody Cat#A303-960A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1462




Experiment Information (Status: Submitting)
BGHcLV41-85BGHcLV41-86
idx13581359
TRCN#_or_BGC#BGC#0001030BGC#0001030
shRNA_or_gRNA_sequenceATGAGACTGTTCGTGAGTGAATGAGACTGTTCGTGAGTGA
PAMTGGTGG
NameMARS_82MARS_82
Sample_IDBGHcLV41-85BGHcLV41-86
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameMARSMARS
qPCR_result32.626.4
Ave_qPCR29.5
RT-qPCR_primer-Fccgctggtttaacatttcgt
RT-qPCR_primer-Rtcagcaactgctggaaaatg
protein_conc34033666
WB_result
Ave_WB
WB_DONE_date
MW101kd
IP
antibody_Cat#A303-960Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Notecheck result befor releasecheck result befor release
ID75557556




Western Blot
Western Blot info was not avaliable




Experiment Status
BGHcLV41-85BGHcLV41-86
Sample_IDBGHcLV41-85BGHcLV41-86
Sample Name
Sample NameMARS-BGHcLV41-85MARS-BGHcLV41-86
RBPMARSMARS
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1298412985




Library-Prep Information
BGHcLV41-85BGHcLV41-86
Sample #3738
Sample NameMARS-BGHcLV41-85MARS-BGHcLV41-86
Sample_Name_Alias
Index Well PositionE05F05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp297301
Peak_Molarity105.00102.00
libSampleQC_DNA_WellDNA_Library_set70/set1/E5.pngDNA_Library_set70/set1/F5.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set70/set1/A11.pngRNA_Library_set70/set1/B11.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-85BGHcLV41-86
RBPMARSMARS
Batch_IDBGHcLV41BGHcLV41
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42624263




Sequencing Information
BGHcLV41-85BGHcLV41-86
Sample_IDBGHcLV41-85BGHcLV41-86
Sample NameMARS-BGHcLV41-85MARS-BGHcLV41-86
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads33,825,28138,104,821
total_aligned_reads31,900,21236,006,390
unique_aligned_reads30,175,72334,080,863
percent_uniqueAligned0.892110.89440
correlation_replicates0.9969620.996962
spikein_reads1,8383,224
percent_spikeins0.000050.00008
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29812982




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database