MARS RNA-Seq in HepG2

MARS CRISPR in HepG2 Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information


RBP	MARS
Cell_Line	HepG2
Method	CRISPR
Exp_Name	MARS-BGHcLV41-HepG2
ENCODE_series_ID
Batch_ID	BGHcLV41
Pool ID	Pool-240314
Local_Set_Name	set70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1	MARS-BGHcLV41-85
Rep2	MARS-BGHcLV41-86
CN1	NT-BGHcLV41-1
CN2	NT-BGHcLV41-2
Rep1_qPCR	32.6
Rep2_qPCR	26.4
Rep1_WB
Rep2_WB
Antibody Cat#	A303-960A
Antibody Lot#	lot # 1
Antibody DCC ID
Status	Submitted
Project	ENCORE2
ID	1462

Experiment Information (Status: Submitting)

	BGHcLV41-85	BGHcLV41-86
idx	1358	1359
TRCN#_or_BGC#	BGC#0001030	BGC#0001030
shRNA_or_gRNA_sequence	ATGAGACTGTTCGTGAGTGA	ATGAGACTGTTCGTGAGTGA
PAM	TGG	TGG
Name	MARS_82	MARS_82
Sample_ID	BGHcLV41-85	BGHcLV41-86
transduction_Date	1/12/24	1/12/24
days	D6	D6
RBP_name	MARS	MARS
qPCR_result	32.6	26.4
Ave_qPCR	29.5
RT-qPCR_primer-F	ccgctggtttaacatttcgt
RT-qPCR_primer-R	tcagcaactgctggaaaatg
protein_conc	3403	3666
WB_result
Ave_WB
WB_DONE_date
MW	101kd
IP
antibody_Cat#	A303-960A	lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note	check result befor release	check result befor release
ID	9603	9604

Western Blot Western Blot info was not avaliable

Experiment Status

	BGHcLV41-85	BGHcLV41-86
Sample_ID	BGHcLV41-85	BGHcLV41-86
Sample Name
Sample Name	MARS-BGHcLV41-85	MARS-BGHcLV41-86
RBP	MARS	MARS
Cell_Line	HepG2	HepG2
Exp UID
Status	Submitting	Submitting
Status_date	2024-09-20	2024-09-20
Project	ENCORE2	ENCORE2
Note
ID	12984	12985

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV41-85	BGHcLV41-86
Sample #	37	38
Sample Name	MARS-BGHcLV41-85	MARS-BGHcLV41-86
Sample_Name_Alias
Index Well Position	E05	F05
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2024-03-01	2024-03-01
Lib_ID	Lib-240301	Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp	297	301
Peak_Molarity	105.00	102.00
libSampleQC_DNA_Well	DNA_Library_set70/set1/E5.png	DNA_Library_set70/set1/F5.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set70/set1/A11.png	RNA_Library_set70/set1/B11.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-03-14	2024-03-14
Sample_ID	BGHcLV41-85	BGHcLV41-86
RBP	MARS	MARS
Batch_ID	BGHcLV41	BGHcLV41
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4262	4263

Sequencing Information

	BGHcLV41-85	BGHcLV41-86
Sample_ID	BGHcLV41-85	BGHcLV41-86
Sample Name	MARS-BGHcLV41-85	MARS-BGHcLV41-86
Pool ID	Pool-240314	Pool-240314
LocalServer_folder	set70	set70
total_reads	33,825,281	38,104,821
total_aligned_reads	31,900,212	36,006,390
unique_aligned_reads	30,175,723	34,080,863
percent_uniqueAligned	0.89211	0.89440
correlation_replicates	0.996962	0.996962
spikein_reads	1,838	3,224
percent_spikeins	0.00005	0.00008
original_ReadLength	151	151
QC_Status	Submitting	Submitting
ID	2981	2982

Data Submission Information DCC submission information was not avaliable

File Information Files of the experiment was not avaliable in the database