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FKBP3 RNA-Seq in HepG2



FKBP3      CRISPR in HepG2      Control:

General Information
RBPFKBP3
Cell_LineHepG2
MethodCRISPR
Exp_NameFKBP3-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1FKBP3-BGHcLV41-75
Rep2FKBP3-BGHcLV41-76
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR73.9
Rep2_qPCR68.4
Rep1_WB76.3
Rep2_WB68.0
Antibody Cat#A302-600A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1460




Experiment Information (Status: Submitting)
BGHcLV41-75BGHcLV41-76
idx13481349
TRCN#_or_BGC#BGC#0001021BGC#0001021
shRNA_or_gRNA_sequenceTCCACGGTCCACGCCCGCTGTCCACGGTCCACGCCCGCTG
PAMTGGTGG
NameFKBP3_90FKBP3_90
Sample_IDBGHcLV41-75BGHcLV41-76
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameFKBP3FKBP3
qPCR_result73.968.4
Ave_qPCR71.1
RT-qPCR_primer-Faaggctcgactggagattga
RT-qPCR_primer-Rtgagttttgcatttggtgga
protein_conc36783900
WB_result76.368.0
Ave_WB72.2
WB_DONE_date1/24/24
MW25kd
IP
antibody_Cat#A302-600Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75437544




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FKBP3Product_ID: A302-600A
Lot_ID: 1
Source: Bethyl Labs
Target Name: FKBP3-human		 FKBP3-HEPG2-CRISPR-A302-600A.png<br>Caption: Western blot following CRISPR against FKBP3 in HepG2 whole cell lysate using FKBP3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FKBP3. FKBP3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 FKBP3-K562-CRISPR-A302-600A.png<br>Caption: Western blot following CRISPR against FKBP3 in K562 whole cell lysate using FKBP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FKBP3. FKBP3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-75BGHcLV41-76
Sample_IDBGHcLV41-75BGHcLV41-76
Sample Name
Sample NameFKBP3-BGHcLV41-75FKBP3-BGHcLV41-76
RBPFKBP3FKBP3
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1298012981




Library-Prep Information
BGHcLV41-75BGHcLV41-76
Sample #3334
Sample NameFKBP3-BGHcLV41-75FKBP3-BGHcLV41-76
Sample_Name_Alias
Index Well PositionA05B05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp295296
Peak_Molarity90.3073.90
libSampleQC_DNA_WellDNA_Library_set70/set1/A5.pngDNA_Library_set70/set1/B5.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set70/set1/E10.pngRNA_Library_set70/set1/F10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-75BGHcLV41-76
RBPFKBP3FKBP3
Batch_IDBGHcLV41BGHcLV41
WB_result76.30068.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42584259




Sequencing Information
BGHcLV41-75BGHcLV41-76
Sample_IDBGHcLV41-75BGHcLV41-76
Sample NameFKBP3-BGHcLV41-75FKBP3-BGHcLV41-76
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads32,166,49643,017,407
total_aligned_reads29,163,78438,949,175
unique_aligned_reads27,601,31836,864,079
percent_uniqueAligned0.858080.85696
correlation_replicates0.9931120.993112
spikein_reads5,1761,078
percent_spikeins0.000160.00003
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29772978




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database