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FEN1 RNA-Seq in HepG2



FEN1      CRISPR in HepG2      Control:

General Information
RBPFEN1
Cell_LineHepG2
MethodCRISPR
Exp_NameFEN1-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1FEN1-BGHcLV41-71
Rep2FEN1-BGHcLV41-72
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR53.0
Rep2_qPCR57.0
Rep1_WB76.2
Rep2_WB81.3
Antibody Cat#A300-255A
Antibody Lot#lot # 3
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1459




Experiment Information (Status: Submitting)
BGHcLV41-71BGHcLV41-72
idx13441345
TRCN#_or_BGC#BGC#0001017BGC#0001017
shRNA_or_gRNA_sequenceGACTACTGTGAGAGTATCCGGACTACTGTGAGAGTATCCG
PAMGGGGGG
NameFEN1_93FEN1_93
Sample_IDBGHcLV41-71BGHcLV41-72
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameFEN1FEN1
qPCR_result53.057.0
Ave_qPCR55.0
RT-qPCR_primer-FACTAAGCGGCTGGTGAAGGTCA
RT-qPCR_primer-RGCAGCATAGACTTTGCCAGCCT
protein_conc22941666
WB_result76.281.3
Ave_WB78.8
WB_DONE_date1/24/23
MW43kd
IP
antibody_Cat#A300-255Alot # 3
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75397540




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FEN1Product_ID: A300-255A
Lot_ID: 3
Source: Bethyl Labs
Target Name: FEN1-human		 FEN1-HEPG2-CRISPR-A300-255A.png<br>Caption: Western blot following CRISPR against FEN1 in HepG2 whole cell lysate using FEN1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FEN1. FEN1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 FEN1-K562-CRISPR-A300-255A.png<br>Caption: Western blot following CRISPR against FEN1 in K562 whole cell lysate using FEN1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FEN1. FEN1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-71BGHcLV41-72
Sample_IDBGHcLV41-71BGHcLV41-72
Sample Name
Sample NameFEN1-BGHcLV41-71FEN1-BGHcLV41-72
RBPFEN1FEN1
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1297812979




Library-Prep Information
BGHcLV41-71BGHcLV41-72
Sample #3132
Sample NameFEN1-BGHcLV41-71FEN1-BGHcLV41-72
Sample_Name_Alias
Index Well PositionG04H04
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp289291
Peak_Molarity77.8064.20
libSampleQC_DNA_WellDNA_Library_set70/set1/G4.pngDNA_Library_set70/set1/H4.png
RIN9.910.0
libSample_RNA_WellRNA_Library_set70/set1/C10.pngRNA_Library_set70/set1/D10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-71BGHcLV41-72
RBPFEN1FEN1
Batch_IDBGHcLV41BGHcLV41
WB_result76.20081.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42564257




Sequencing Information
BGHcLV41-71BGHcLV41-72
Sample_IDBGHcLV41-71BGHcLV41-72
Sample NameFEN1-BGHcLV41-71FEN1-BGHcLV41-72
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads41,051,23352,317,218
total_aligned_reads37,955,62349,439,364
unique_aligned_reads35,906,02046,750,360
percent_uniqueAligned0.874660.89359
correlation_replicates0.9984920.998492
spikein_reads4,54211,468
percent_spikeins0.000110.00022
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29752976




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database