FDPS      CRISPR in HepG2      Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information
RBPFDPS
Cell_LineHepG2
MethodCRISPR
Exp_NameFDPS-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1FDPS-BGHcLV41-69
Rep2FDPS-BGHcLV41-70
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR53.8
Rep2_qPCR59.3
Rep1_WB70.8
Rep2_WB77.0
Antibody Cat#A305-045A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1458




Experiment Information (Status: Submitting)
BGHcLV41-69BGHcLV41-70
idx13421343
TRCN#_or_BGC#BGC#0001015BGC#0001015
shRNA_or_gRNA_sequenceGATTCATCCCTTACCCGCCGGATTCATCCCTTACCCGCCG
PAMGGGGGG
NameFDPS_95FDPS_95
Sample_IDBGHcLV41-69BGHcLV41-70
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameFDPSFDPS
qPCR_result53.859.3
Ave_qPCR56.6
RT-qPCR_primer-FCTTTCTTCCTGGTGGCAGATGAC
RT-qPCR_primer-RAGAGCTTCAGCAGGCGGTAGAT
protein_conc27122448
WB_result70.877.0
Ave_WB73.9
WB_DONE_date1/29/24
MW48kd
IP
antibody_Cat#A305-045Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID95859586




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FDPSProduct_ID: A305-045A
Lot_ID: 1
Source: Bethyl Labs
Target Name: FDPS-human		 FDPS-HEPG2-CRISPR-A305-045A.png<br>Caption: Western blot following CRISPR against FDPS in HepG2 whole cell lysate using FDPS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FDPS. FDPS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 FDPS-K562-CRISPR-A305-045A.png<br>Caption: Western blot following CRISPR against FDPS in K562 whole cell lysate using FDPS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FDPS. FDPS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-69BGHcLV41-70
Sample_IDBGHcLV41-69BGHcLV41-70
Sample Name
Sample NameFDPS-BGHcLV41-69FDPS-BGHcLV41-70
RBPFDPSFDPS
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1297612977




Library-Prep Information
BGHcLV41-69BGHcLV41-70
Sample #2930
Sample NameFDPS-BGHcLV41-69FDPS-BGHcLV41-70
Sample_Name_Alias
Index Well PositionE04F04
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp296297
Peak_Molarity82.70102.00
libSampleQC_DNA_WellDNA_Library_set70/set1/E4.pngDNA_Library_set70/set1/F4.png
RIN10.09.9
libSample_RNA_WellRNA_Library_set70/set1/A10.pngRNA_Library_set70/set1/B10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-69BGHcLV41-70
RBPFDPSFDPS
Batch_IDBGHcLV41BGHcLV41
WB_result70.80077.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42544255




Sequencing Information
BGHcLV41-69BGHcLV41-70
Sample_IDBGHcLV41-69BGHcLV41-70
Sample NameFDPS-BGHcLV41-69FDPS-BGHcLV41-70
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads38,158,92935,862,025
total_aligned_reads35,046,83934,023,517
unique_aligned_reads33,144,42032,141,370
percent_uniqueAligned0.868590.89625
correlation_replicates0.9980570.998057
spikein_reads2,1003,358
percent_spikeins0.000060.00009
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29732974




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database