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FAM98A RNA-Seq in HepG2



FAM98A      CRISPR in HepG2      Control:

General Information
RBPFAM98A
Cell_LineHepG2
MethodCRISPR
Exp_NameFAM98A-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1FAM98A-BGHcLV41-65
Rep2FAM98A-BGHcLV41-66
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR29.2
Rep2_qPCR32.7
Rep1_WB67.0
Rep2_WB58.3
Antibody Cat#A305-817A-M
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1457




Experiment Information (Status: Submitting)
BGHcLV41-65BGHcLV41-66
idx13381339
TRCN#_or_BGC#BGC#0001011BGC#0001011
shRNA_or_gRNA_sequenceATGATTACTACTCACCCAGTATGATTACTACTCACCCAGT
PAMGGGGGG
NameFAM98A_81FAM98A_81
Sample_IDBGHcLV41-65BGHcLV41-66
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameFAM98AFAM98A
qPCR_result29.232.7
Ave_qPCR31.0
RT-qPCR_primer-Fccaagccatagccaatgaat
RT-qPCR_primer-Rgaacgtttcggctggtaaac
protein_conc24612336
WB_result67.058.3
Ave_WB62.7
WB_DONE_date1/29/24
MW55kd
IP
antibody_Cat#A305-817A-Mlot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID75337534




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FAM98AProduct_ID: A305-817A-M
Lot_ID: 1
Source: Bethyl Labs
Target Name: FAM98A-human		 FAM98A-HEPG2-CRISPR-A305-817A-M.png<br>Caption: Western blot following CRISPR against FAM98A in HepG2 whole cell lysate using FAM98A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FAM98A. FAM98A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 FAM98A-K562-CRISPR-A305-817A.png<br>Caption: Western blot following CRISPR against FAM98A in K562 whole cell lysate using FAM98A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FAM98A. FAM98A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-65BGHcLV41-66
Sample_IDBGHcLV41-65BGHcLV41-66
Sample Name
Sample NameFAM98A-BGHcLV41-65FAM98A-BGHcLV41-66
RBPFAM98AFAM98A
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1297412975




Library-Prep Information
BGHcLV41-65BGHcLV41-66
Sample #2728
Sample NameFAM98A-BGHcLV41-65FAM98A-BGHcLV41-66
Sample_Name_Alias
Index Well PositionC04D04
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp300305
Peak_Molarity99.8088.50
libSampleQC_DNA_WellDNA_Library_set70/set1/C4.pngDNA_Library_set70/set1/D4.png
RIN9.89.9
libSample_RNA_WellRNA_Library_set70/set1/G9.pngRNA_Library_set70/set1/H9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-65BGHcLV41-66
RBPFAM98AFAM98A
Batch_IDBGHcLV41BGHcLV41
WB_result67.00058.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42524253




Sequencing Information
BGHcLV41-65BGHcLV41-66
Sample_IDBGHcLV41-65BGHcLV41-66
Sample NameFAM98A-BGHcLV41-65FAM98A-BGHcLV41-66
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads32,659,43039,922,950
total_aligned_reads29,826,71637,824,830
unique_aligned_reads28,229,28135,742,736
percent_uniqueAligned0.864350.89529
correlation_replicates0.9968280.996828
spikein_reads2,8961,400
percent_spikeins0.000090.00004
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29712972




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database