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EIF3J RNA-Seq in HepG2



EIF3J      CRISPR in HepG2      Control:

General Information
RBPEIF3J
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF3J-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF3J-BGHcLV41-31
Rep2EIF3J-BGHcLV41-32
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR61.0
Rep2_qPCR57.0
Rep1_WB90.9
Rep2_WB94.2
Antibody Cat#A301-746A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1451




Experiment Information (Status: Submitting)
BGHcLV41-31BGHcLV41-32
idx13001301
TRCN#_or_BGC#BGC#0000969BGC#0000969
shRNA_or_gRNA_sequenceTCACACCCGGCTCGAGATGGTCACACCCGGCTCGAGATGG
PAMCGGCGG
NameEIF3J_90EIF3J_90
Sample_IDBGHcLV41-31BGHcLV41-32
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameEIF3JEIF3J
qPCR_result61.057.0
Ave_qPCR59.0
RT-qPCR_primer-Ftgactgtgctttgcagtgaa
RT-qPCR_primer-Rtgccagatcatctttcatgg
protein_conc34513410
WB_result90.994.2
Ave_WB92.5
WB_DONE_date1/24/24
MW29KD
IP
antibody_Cat#A301-746Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID74937494




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF3JProduct_ID: A301-746A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF3J-human		 EIF3J-HEPG2-CRISPR-A301-746A.png<br>Caption: Western blot following CRISPR against EIF3J in HepG2 whole cell lysate using EIF3J specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3J. EIF3J protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EIF3J-K562-CRISPR-A301-746A.png<br>Caption: Western blot following CRISPR against EIF3J in K562 whole cell lysate using EIF3J specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3J. EIF3J protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-31BGHcLV41-32
Sample_IDBGHcLV41-31BGHcLV41-32
Sample Name
Sample NameEIF3J-BGHcLV41-31EIF3J-BGHcLV41-32
RBPEIF3JEIF3J
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1296212963




Library-Prep Information
BGHcLV41-31BGHcLV41-32
Sample #1516
Sample NameEIF3J-BGHcLV41-31EIF3J-BGHcLV41-32
Sample_Name_Alias
Index Well PositionG02H02
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp286289
Peak_Molarity70.7067.20
libSampleQC_DNA_WellDNA_Library_set70/set1/G2.pngDNA_Library_set70/set1/H2.png
RIN9.910.0
libSample_RNA_WellRNA_Library_set70/set1/C8.pngRNA_Library_set70/set1/D8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-31BGHcLV41-32
RBPEIF3JEIF3J
Batch_IDBGHcLV41BGHcLV41
WB_result90.90094.200
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42404241




Sequencing Information
BGHcLV41-31BGHcLV41-32
Sample_IDBGHcLV41-31BGHcLV41-32
Sample NameEIF3J-BGHcLV41-31EIF3J-BGHcLV41-32
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads45,991,51639,717,006
total_aligned_reads41,132,43835,159,719
unique_aligned_reads38,805,99533,098,455
percent_uniqueAligned0.843760.83336
correlation_replicates0.9952100.995210
spikein_reads7,0048,854
percent_spikeins0.000150.00022
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29592960




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database