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EIF3E RNA-Seq in HepG2



EIF3E      CRISPR in HepG2      Control:

General Information
RBPEIF3E
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF3E-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF3E-BGHcLV41-29
Rep2EIF3E-BGHcLV41-30
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR69.4
Rep2_qPCR70.5
Rep1_WB59.8
Rep2_WB53.6
Antibody Cat#A302-985A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1450




Experiment Information (Status: Submitting)
BGHcLV41-29BGHcLV41-29BGHcLV41-30BGHcLV41-30
idx1298129812991299
TRCN#_or_BGC#BGC#0000968BGC#0000968BGC#0000968BGC#0000968
shRNA_or_gRNA_sequenceCAAAATTCCAGTACGAATGTCAAAATTCCAGTACGAATGTCAAAATTCCAGTACGAATGTCAAAATTCCAGTACGAATGT
PAMGGGGGGGGGGGG
NameEIF3E_79EIF3E_79EIF3E_79EIF3E_79
Sample_IDBGHcLV41-29BGHcLV41-29BGHcLV41-30BGHcLV41-30
transduction_Date1/12/241/12/241/12/241/12/24
daysD6D6D6D6
RBP_nameEIF3EEIF3EEIF3EEIF3E
qPCR_result69.469.470.570.5
Ave_qPCR70.070.0
RT-qPCR_primer-Ftcaggcagaaacagaaccaatcaggcagaaacagaaccaa
RT-qPCR_primer-Rgtccgccaggtagtcaaagagtccgccaggtagtcaaaga
protein_conc3478347835453545
WB_result59.854.753.652.1497
Ave_WB56.7
WB_DONE_date1/29/2411/7/24
MW52kd52kd
IP
antibody_Cat#A302-985AA302-984Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID7489749174907492




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF3EProduct_ID: A302-985A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF3E-human		 EIF3E-HEPG2-CRISPR-A302-985A.png<br>Caption: Western blot following CRISPR against EIF3E in HepG2 whole cell lysate using EIF3E specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3E. EIF3E protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-29BGHcLV41-30
Sample_IDBGHcLV41-29BGHcLV41-30
Sample Name
Sample NameEIF3E-BGHcLV41-29EIF3E-BGHcLV41-30
RBPEIF3EEIF3E
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1296012961




Library-Prep Information
BGHcLV41-29BGHcLV41-30
Sample #1314
Sample NameEIF3E-BGHcLV41-29EIF3E-BGHcLV41-30
Sample_Name_Alias
Index Well PositionE02F02
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp294299
Peak_Molarity92.5094.70
libSampleQC_DNA_WellDNA_Library_set70/set1/E2.pngDNA_Library_set70/set1/F2.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set70/set1/A8.pngRNA_Library_set70/set1/B8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-29BGHcLV41-30
RBPEIF3EEIF3E
Batch_IDBGHcLV41BGHcLV41
WB_result59.80053.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42384239




Sequencing Information
BGHcLV41-29BGHcLV41-30
Sample_IDBGHcLV41-29BGHcLV41-30
Sample NameEIF3E-BGHcLV41-29EIF3E-BGHcLV41-30
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads35,997,02842,179,093
total_aligned_reads32,559,22038,080,701
unique_aligned_reads30,709,43835,894,943
percent_uniqueAligned0.853110.85101
correlation_replicates0.9984000.998400
spikein_reads3,2046,186
percent_spikeins0.000090.00015
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29572958




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database