EEF2K      CRISPR in HepG2      Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information
RBPEEF2K
Cell_LineHepG2
MethodCRISPR
Exp_NameEEF2K-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EEF2K-BGHcLV41-21
Rep2EEF2K-BGHcLV41-22
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR50.9
Rep2_qPCR57.1
Rep1_WB
Rep2_WB
Antibody Cat#A301-686A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1447




Experiment Information (Status: Submitting)
BGHcLV41-21BGHcLV41-22
idx12881289
TRCN#_or_BGC#BGC#0000951BGC#0000951
shRNA_or_gRNA_sequenceCCCAAGTAAGGACCCTCTCTCCCAAGTAAGGACCCTCTCT
PAMgggggg
NameEEF2K_72EEF2K_72
Sample_IDBGHcLV41-21BGHcLV41-22
transduction_Date6/10/236/10/23
daysD6D6
RBP_nameEEF2KEEF2K
qPCR_result50.957.1
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc22061884
WB_result0.00.0
Ave_WB0.0
WB_DONE_date2/5/24
MW82KD
IP
antibody_Cat#A301-686Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Notecheck result befor release check result befor release
ID95299530




Western Blot
Western Blot info was not avaliable




Experiment Status
BGHcLV41-21BGHcLV41-22
Sample_IDBGHcLV41-21BGHcLV41-22
Sample Name
Sample NameEEF2K-BGHcLV41-21EEF2K-BGHcLV41-22
RBPEEF2KEEF2K
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1295412955




Library-Prep Information
BGHcLV41-21BGHcLV41-22
Sample #78
Sample NameEEF2K-BGHcLV41-21EEF2K-BGHcLV41-22
Sample_Name_Alias
Index Well PositionG01H01
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp300300
Peak_Molarity51.3059.30
libSampleQC_DNA_WellDNA_Library_set70/set1/G1.pngDNA_Library_set70/set1/H1.png
RIN9.69.7
libSample_RNA_WellRNA_Library_set70/set1/C7.pngRNA_Library_set70/set1/D7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-21BGHcLV41-22
RBPEEF2KEEF2K
Batch_IDBGHcLV41BGHcLV41
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42324233




Sequencing Information
BGHcLV41-21BGHcLV41-22
Sample_IDBGHcLV41-21BGHcLV41-22
Sample NameEEF2K-BGHcLV41-21EEF2K-BGHcLV41-22
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads61,439,50443,652,689
total_aligned_reads56,371,46439,898,662
unique_aligned_reads53,236,93537,686,302
percent_uniqueAligned0.866490.86332
correlation_replicates0.9997900.999790
spikein_reads8,33814,336
percent_spikeins0.000140.00033
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29512952




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database