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DYNC1I2 RNA-Seq in HepG2



DYNC1I2      CRISPR in HepG2      Control:

General Information
RBPDYNC1I2
Cell_LineHepG2
MethodCRISPR
Exp_NameDYNC1I2-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1DYNC1I2-BGHcLV41-15
Rep2DYNC1I2-BGHcLV41-16
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR72.5
Rep2_qPCR69.3
Rep1_WB90.6
Rep2_WB84.5
Antibody Cat#A304-529A
Antibody Lot#lot# 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1446




Experiment Information (Status: Submitting)
BGHcLV41-15BGHcLV41-16
idx12821283
TRCN#_or_BGC#BGC#0000938BGC#0000938
shRNA_or_gRNA_sequenceATTACTGTACCTAGATCCCAATTACTGTACCTAGATCCCA
PAMCGGCGG
NameDYNC1I2_82DYNC1I2_82
Sample_IDBGHcLV41-15BGHcLV41-16
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameDYNC1I2DYNC1I2
qPCR_result72.569.3
Ave_qPCR70.9
RT-qPCR_primer-Fgggagattggatttgtggaa
RT-qPCR_primer-Rcttcagaatcacccacagca
protein_conc29263336
WB_result90.684.5
Ave_WB
WB_DONE_date1/26/24
MW71KD
IP
antibody_Cat#A304-529Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID74737474




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DYNC1I2Product_ID: A304-529A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DYNC1I2-human		 DYNC1I2-HEPG2-CRISPR-A304-529A.png<br>Caption: Western blot following CRISPR against DYNC1I2 in HepG2 whole cell lysate using DYNC1I2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DYNC1I2. DYNC1I2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 DYNC1I2-K562-CRISPR-A304-529A.png<br>Caption: Western blot following CRISPR against DYNC1I2 in K562 whole cell lysate using DYNC1I2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DYNC1I2. DYNC1I2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-15BGHcLV41-16
Sample_IDBGHcLV41-15BGHcLV41-16
Sample Name
Sample NameDYNC1I2-BGHcLV41-15DYNC1I2-BGHcLV41-16
RBPDYNC1I2DYNC1I2
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1295212953




Library-Prep Information
BGHcLV41-15BGHcLV41-16
Sample #56
Sample NameDYNC1I2-BGHcLV41-15DYNC1I2-BGHcLV41-16
Sample_Name_Alias
Index Well PositionE01F01
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp295299
Peak_Molarity65.6066.10
libSampleQC_DNA_WellDNA_Library_set70/set1/E1.pngDNA_Library_set70/set1/F1.png
RIN9.69.7
libSample_RNA_WellRNA_Library_set70/set1/A7.pngRNA_Library_set70/set1/B7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-15BGHcLV41-16
RBPDYNC1I2DYNC1I2
Batch_IDBGHcLV41BGHcLV41
WB_result90.60084.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42304231




Sequencing Information
BGHcLV41-15BGHcLV41-16
Sample_IDBGHcLV41-15BGHcLV41-16
Sample NameDYNC1I2-BGHcLV41-15DYNC1I2-BGHcLV41-16
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads43,815,19438,615,155
total_aligned_reads38,576,29133,442,434
unique_aligned_reads36,303,57831,553,072
percent_uniqueAligned0.828560.81712
correlation_replicates0.9862810.986281
spikein_reads5,5904,410
percent_spikeins0.000130.00011
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29492950




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database