MORC2 RNA-Seq in HepG2

General Information


RBP	MORC2
Cell_Line	HepG2
Method	CRISPR
Exp_Name	MORC2-BGHcLV20-HepG2
ENCODE_series_ID	ENCSR430MCS
Batch_ID	BGHcLV20
Pool ID	Pool-200928
Local_Set_Name	set45_H
ENCODE_KD_Exp_ID	ENCSR444IOJ
ENCODE_CN_Exp_ID	ENCSR306EWE
Rep1	MORC2-BGHcLV20-13
Rep2	MORC2-BGHcLV20-14
CN1	NT-BGHcLV20-1
CN2	NT-BGHcLV20-2
Rep1_qPCR	0.0
Rep2_qPCR	0.0
Rep1_WB	61.6
Rep2_WB	63.0
Antibody Cat#	PA5-51172
Antibody Lot#	lot#UF2796314
Antibody DCC ID	ENCAB179RUB
Status	Released
Project	ENCODE4
ID	76

Experiment Information (Status: Released)

	BGHcLV20-13	BGHcLV20-14
idx	563	564
TRCN#_or_BGC#	BGC#0000471	BGC#0000471
shRNA_or_gRNA_sequence	AGATTGGGCAGTACGGGAAT	AGATTGGGCAGTACGGGAAT
PAM	GGG	GGG
Name	MORC2_91	MORC2_91
Sample_ID	BGHcLV20-13	BGHcLV20-14
transduction_Date	12/10/19	12/10/19
days	D6	D6
RBP_name	MORC2	MORC2
qPCR_result	0.0	0.0
Ave_qPCR	0.0
RT-qPCR_primer-F	GCAGAGAAGCCGATGTCAAG
RT-qPCR_primer-R	CAGATCCCGGTGTTCAATGT
protein_conc	4738	4937
WB_result	61.6	63.0
Ave_WB	62.3
WB_DONE_date	8/10/20	JESS
MW	117.8kd
IP	Rabbit / I
antibody_Cat#	PA5-51172	lot#UF2796314
Antibody DCC ID	ENCAB179RUB
submitted_to_DCC_date	11/16/20
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date	09/24/20	09/24/20
repeat_library
Note
ID	6718	6719

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
MORC2	Product_ID: PA5-51172 Lot_ID: UF2796314 Source: Thermo Fisher Target Name: MORC2-human

Experiment Status

	BGHcLV20-13	BGHcLV20-14
Sample_ID	BGHcLV20-13	BGHcLV20-14
Sample Name
Sample Name	MORC2-BGHcLV20-13	MORC2-BGHcLV20-14
RBP	MORC2	MORC2
Cell_Line	HepG2	HepG2
Exp UID
Status	Released	Released
Status_date	2021-03-18	2021-03-18
Project	ENCODE4	ENCODE4
Note
ID	5813	5814

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV20-13	BGHcLV20-14
Sample #	63	64
Sample Name	MORC2-BGHcLV20-13	MORC2-BGHcLV20-14
Sample_Name_Alias
Index Well Position	A08	B08
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2020-09-24	2020-09-24
Lib_ID	Lib-200924	Lib-200924
Tecan_Location
Tecan
Tecan_date
Size_bp	310	310
Peak_Molarity	90.80	97.90
libSampleQC_DNA_Well	DNA_Library_set45/set1/A8.png	DNA_Library_set45/set1/B8.png
RIN	9.5	9.4
libSample_RNA_Well	RNA_Library_set45/set1/A8.png	RNA_Library_set45/set1/B8.png
SampleQC_method	TapeStation	TapeStation
SampleQC_date	2020-09-22	2020-09-22
Sample_ID	BGHcLV20-13	BGHcLV20-14
RBP	MORC2	MORC2
Batch_ID	BGHcLV20	BGHcLV20
WB_result	61.600	63.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCODE4	ENCODE4
ID	837	838

Sequencing Information

	BGHcLV20-13	BGHcLV20-14
Sample_ID	BGHcLV20-13	BGHcLV20-14
Sample Name	MORC2-BGHcLV20-13	MORC2-BGHcLV20-14
Pool ID	Pool-200928	Pool-200928
LocalServer_folder	set45_H	set45_H
total_reads	100,590,784	106,425,254
total_aligned_reads	96,868,596	98,379,928
unique_aligned_reads	91,289,932	92,647,074
percent_uniqueAligned	0.90754	0.87054
correlation_replicates	0.991853	0.991853
spikein_reads	101,710	74,956
percent_spikeins	0.00101	0.00070
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	468	469

Data Submission Information

	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-14	BGHcLV20-14	BGHcLV20-14	BGHcLV20-14
ENCODE_alias	brenton-graveley:MORC2-BGHcLV20	brenton-graveley:MORC2-BGHcLV20-13	brenton-graveley:L-MORC2-BGHcLV20-13	brenton-graveley:MORC2-BGHcLV20-13_S57_R1_001.fastq.gz	brenton-graveley:MORC2-BGHcLV20-13_S57_R2_001.fastq.gz	brenton-graveley:MORC2-BGHcLV20-HepG2	brenton-graveley:MORC2-BGHcLV20-14	brenton-graveley:L-MORC2-BGHcLV20-14	brenton-graveley:MORC2-BGHcLV20-14_S58_R1_001.fastq.gz	brenton-graveley:MORC2-BGHcLV20-14_S58_R2_001.fastq.gz
ENCODE_accession	ENCSR444IOJ	ENCBS581RKB	ENCLB971OMN	ENCFF453GKS	ENCFF813ILG	ENCSR430MCS	ENCBS300KME	ENCLB115VAG	ENCFF976KNT	ENCFF008MCE
object_type	experiment	biosample	library	file_fastq1	file_fastq2	gene_silencing_series	biosample	library	file_fastq1	file_fastq2
assay_type	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq	knockdown followed by RNA-seq
Sample_ID	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-13	BGHcLV20-14	BGHcLV20-14	BGHcLV20-14	BGHcLV20-14
Sample Name	MORC2-BGHcLV20-13	MORC2-BGHcLV20-13	MORC2-BGHcLV20-13	MORC2-BGHcLV20-13	MORC2-BGHcLV20-13	MORC2-BGHcLV20-13	MORC2-BGHcLV20-14	MORC2-BGHcLV20-14	MORC2-BGHcLV20-14	MORC2-BGHcLV20-14
Selected	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Status	Released	Released	Released	Released	Released	Released	Released	Released	Released	Released
Status_Date	2021-03-18	2021-03-18	2021-03-18	2021-03-18	2021-03-18	2022-03-02	2021-03-18	2021-03-18	2021-03-18	2021-03-18
Project	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4	ENCODE4
		LV45H_biosample_protocol	LV45H_library_protocol				LV45H_biosample_protocol	LV45H_library_protocol
protocol_URL		MORC2-BGHcLV20-13.pdf	L-MORC2-BGHcLV20-13.pdf				MORC2-BGHcLV20-14.pdf	L-MORC2-BGHcLV20-14.pdf
ID	2083	2121	2191	2261	2331	7259	2122	2192	2262	2332

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
MORC2-BGHcLV20-13_S57_R1_001.fastq.gz	ENCODE_DATA/set45_H/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	468	NovaSeq6000	100	paired-ended	1				NT-BGHcLV20-1_S51_R1_001.fastq.gz,NT-BGHcLV20-2_S52_R1_001.fastq.gz	ENCODE4	1312
MORC2-BGHcLV20-13_S57_R2_001.fastq.gz	ENCODE_DATA/set45_H/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	468	NovaSeq6000	100	paired-ended	2				NT-BGHcLV20-1_S51_R2_001.fastq.gz,NT-BGHcLV20-2_S52_R2_001.fastq.gz	ENCODE4	1332
MORC2-BGHcLV20-13_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13_S57_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGHcLV20-13_S57_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	24853
MORC2-BGHcLV20-13_quant.sf	ENCODE_DATA/set45_H/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13_S57_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGHcLV20-13_S57_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	24873
MORC2-BGHcLV20-13_Signal.Unique.strand+.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13/Aligned.sortedByCoord.out.bam		ENCORE2	25923
MORC2-BGHcLV20-13_Signal.Unique.strand-.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13/Aligned.sortedByCoord.out.bam		ENCORE2	25943
MORC2-BGHcLV20-13_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13/Aligned.sortedByCoord.out.bam		ENCORE2	25963
MORC2-BGHcLV20-13_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	468					GRCh38	V40	MORC2-BGHcLV20-13/Aligned.sortedByCoord.out.bam		ENCORE2	25983
MORC2-BGHcLV20-14_S58_R1_001.fastq.gz	ENCODE_DATA/set45_H/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	469	NovaSeq6000	100	paired-ended	1				NT-BGHcLV20-1_S51_R1_001.fastq.gz,NT-BGHcLV20-2_S52_R1_001.fastq.gz	ENCODE4	1313
MORC2-BGHcLV20-14_S58_R2_001.fastq.gz	ENCODE_DATA/set45_H/fastq	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	469	NovaSeq6000	100	paired-ended	2				NT-BGHcLV20-1_S51_R2_001.fastq.gz,NT-BGHcLV20-2_S52_R2_001.fastq.gz	ENCODE4	1333
MORC2-BGHcLV20-14_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14_S58_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGHcLV20-14_S58_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	24854
MORC2-BGHcLV20-14_quant.sf	ENCODE_DATA/set45_H/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14_S58_R1_001.filtered.trimmed.paired.fastq.gz,MORC2-BGHcLV20-14_S58_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	24874
MORC2-BGHcLV20-14_Signal.Unique.strand+.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14/Aligned.sortedByCoord.out.bam		ENCORE2	25924
MORC2-BGHcLV20-14_Signal.Unique.strand-.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14/Aligned.sortedByCoord.out.bam		ENCORE2	25944
MORC2-BGHcLV20-14_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14/Aligned.sortedByCoord.out.bam		ENCORE2	25964
MORC2-BGHcLV20-14_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set45_H/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	469					GRCh38	V40	MORC2-BGHcLV20-14/Aligned.sortedByCoord.out.bam		ENCORE2	25984